| 货号 | IC835G-025 |
| 别名 | Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA |
| 反应种属 | Human/Mouse |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 836 (Human); 12367 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | KLH-conjugated human Caspase-3 synthetic peptide CRGTELDCGIETD Accession # U26943 |
| 生物活性 | Human, Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Cleaved Caspase‑3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line untreated (open histogram) or treated with 1 μM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC835G, filled histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules. |
