Human/Mouse/Rat p70S6 Kinase Phycoerythrin MAb (Cl 215247) (25 TESTS)
货号：
IC8962P-025 基本售价：
2111.7 元 规格：
25 Tests
产品信息
概述| 货号 | IC8962P-025 |
| 别名 | EC 2.7.11; EC 2.7.11.1; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6 kinase, alpha 1,70 kDa ribosomal protein S6 kinase 1; p70 S6 kinase, alpha 2; p70 S6KA; p70 S6K-alpha; p70(S6K)-alpha; p70-alpha; p70-S6K; P70S6K1; PS6K; ribosomal protein S6 kinase beta-1; Ribosomal protein S6 kinase I; ribosomal protein S6 kinase, 70kD, polypeptide 1; ribosomal protein S6 kinase, 70kDa, polypeptide 1; RPS6KB1; S6K; S6K1; S6K1p70-S6K 1; S6K-beta-1; serine/threonine kinase 14 alpha; Serine/threonine-protein kinase 14A; STK14A |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects human, mouse, and rat p70 S6 Kinase and p85 S6 Kinase in Western blots. Reactivity with beta isoforms of p70 S6 Kinase is unknown. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 10 µL/106cells
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 6198 (Human); 72508 (Mouse); 83840 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human p70 S6 Kinase
Accession # M60725 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Phycoerythrin |
| 背景 | p70 S6 Kinase (p70S6K) is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues. p70S6K is activated by serum stimulation and this activation is inhibited by wortmannin and rapamycin. p70S6K activity undergoes changes during the cell cycle and increases 20‑fold in G1 cells released from G0. p70S6K activation requires sequential phosphorylations at proline-directed residues in the putative autoinhibitory pseudosubstrate domain, as well asT389, a site phosphorylated by phosphoinositide-dependent kinase 1 (PDK1) (1-3). Over amino acids (aa) 1-502, human and mouse share 99% aa sequence identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
- Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.
- Fenton, T.R. and I.T. Gout (2011) Int. J. Biochem. Cell Biol. 43:47.
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参考图片Detection of p70 S6 Kinase in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human/Mouse/Rat p70 S6 Kinase PE‑conjugated Monoclonal Antibody (Catalog # IC8962P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
