货号 | IC20571G-025 |
简介 | Recombinant Monoclonal Antibody |
别名 | Cytokine ML-1; IL-17Finterleukin-17F; IL24; IL-24; interleukin 17F; Interleukin-24; ML-1; ML1interleukin-24 | 全称 | Interleukin 17F |
应用 | Intracellular Staining by Flow Cytometry |
目标/特异性 | Detects mouse IL-17F in direct ELISAs. |
使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 112744 (Human); 257630 (Mouse); 301291 (Rat) |
纯化方式 | Protein A or G purified from cell culture supernatant |
免疫原 | E. coli-derived recombinant mouse IL-17F Ala21-Val153 Accession # Q7TNI7 |
生物活性 | Mouse |
标记 | Alexa Fluor 488 |
背景 | IL-17F, also called ML-1, is a 21-33 kDa member of the IL-17 family of protein. It is one of six glycoproteins with conserved cysteines that form a cystine knot structure. IL-17F is expressed by multiple cell types, including bronchial epthelium, neutrophils (likely), monocytes, mast cells, colonic epithelium, gamma δ T cells, CD4+ Th17 cells, group 3 (NKp46- ROR gamma t) innate lymphoid cells, NKT cells and CD8+ Tc17 cells. IL-17F is known to form disulfide-linked homodimers, and covalent heterodimes with IL-17A. Notable, the IL-17A and IL-17F homodimers, plus the IL-17A:F heterodimer use the same receptor combination (IL-17RA:IL-17RC), but elicit somewhat different outcomes. For instance, on macrophages, IL-17A induces IL-9, KC and GM-CSF secretion, while IL-17F does not. Both molecules, however, induce the secretion of the same molecules (IL-9, KC, and GM-CSF) by colonic epithelium. Mouse IL-17F has a long form and a short form, which have alternate start sites but identical lengths. Over amino acids (aa) 21-153, mouse IL-17F shares 88% and 56% aa sequence identity with rat and human IL-17F, respectively. |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 |
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Detection of IL‑17F in Mouse Splenocytes Stimulated to Induce Th17 Cells by Flow Cytometry. Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 APC‑conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rabbit Anti-Mouse IL‑17F Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC20571G) or (B) Normal Rabbit IgG Alexa Fluor 488 Control (Catalog # IC105G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |