| 货号 | IC8086R-025 |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | Gm1365; toll-like receptor 12 | 全称 | Toll-like Receptor 12 |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects mouse TLR12 in direct ELISAs. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 384059 (Mouse) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse TLR12 Thr22-Lys709 Accession # Q6QNU9 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 647 |
| 背景 | Toll-like receptors (TLRs) are type I transmembrane proteins that activate the immune system in response to molecular patterns derived from microbial pathogens. Some TLRs are expressed on the cell surface (TLR1, 2, 4, 5, 6, 10), while others are found in endosomal structures (TLR3, 7, 8, 9, 11, 12, 13). TLRs contain a large number of leucine-rich repeats (LRRs) in their ectodomains and a cytoplasmic tail with one signal transducing Toll/IL-1 receptor (TIR) domain (1, 2). Mature mouse TLR12 is an approximately 100 kDa glycoprotein that belongs to the TLR11 family of TLRs. It consists of a 688 amino acid (aa) ectodomain with 17 LRRs, a 21 aa transmembrane segment, and a 176 aa cytoplasmic region (3). Over aa21-709, mouse TLR12 shares 80% aa sequence identity with rat TLR12. There is no human counterpart. TLR12 is expressed in dendritic cells, macrophages, and neurons, and in the uterus, liver, kidney, and bladder (3-6). It is up-regulated in the adipose tissue of obesity-prone ob/ob mice fed a high fat diet (7). The intracellular trafficking and sorting of several TLRs (including TLR12) is dependent on the endoplasmic reticulum resident protein UNC93B1 (8, 9). TLR12 can associate into homodimers as well as heterodimers with TLR11 (4). TLR11 can additionally heterodimerize with TLR3 and TLR7 (4, 8). TLR12 is required for the inflammatory response against uropathogenic bacteria and Toxoplasma gondii (3, 4). It directly binds Profilin from T. gondii, and it cooperates with TLR11 in protecting against T. gondiiinfectionin vivo(4). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of TLR12 in HEK293 Human Cell Line Transfected with Mouse TLR12 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse TLR12 and eGFP was stained with and either (A) Rabbit Anti-Mouse TLR12 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC8086R) or (B) Normal Rabbit IgG Alexa Fluor 647 Control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules. |
