| 货号 | IC8889G-025 |
| 简介 | Recombinant Monoclonal Antibody |
| 别名 | eomesodermin (Xenopus laevis) homolog; Eomesodermin; t box, brain, 2; T-box brain protein 2; T-box brain2; TBR2; TBR-2; TBR2eomesodermin homolog; T-brain-2 | 全称 | Eomesodermin Homolog |
| 应用 | Intracellular Staining by Flow Cytometry |
| 目标/特异性 | Detects mouse EOMES in direct ELISAs. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 5 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8320 (Human); 13813 (Mouse); 316052 (Rat) |
| 纯化方式 | Protein A or G purified from cell culture supernatant |
| 免疫原 | E. coli-derived recombinant mouse EOMES Met1-Ser126 Accession # O54839 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | EOMES (Eomesodermin), also TBR2, is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Mouse EOMES is 707 amino acids (aa) in length. It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T box (aa 278-458). There is one isoform that shows a deletion of aa 463-481. Over aa 1‑126, mouse EOMES shares 76% aa sequence identity with human EOMES. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of EOMES in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rabbit Anti-Mouse EOMES Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8889G) or (B) Normal Rabbit IgG Alexa Fluor 488 Control (Catalog # IC105G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules. |
