| 货号 | FAB9215G-025UG |
| 别名 | alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140a antigen; CD140a; EC 2.7.10; EC 2.7.10.1; MGC74795; PDGF R alpha; PDGFR2; PDGFRA; PDGFRA/BCR fusion; PDGF-R-alpha; platelet-derived growth factor receptor, alpha polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alphafusion protein; RHEPDGFRA | 全称 | Platelet-derived Growth Factor Receptor alpha |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse PDGF R alpha in direct ELISAs. |
| 使用方法 | Flow Cytometry: 0.5 µg/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5156 (Human); 18595 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse PDGF R alpha Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala), predicted Accession # P26618 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | PDGF R alpha (Platelet-DerivedGrowth Factor Receptor alpha), also known as CD140a, is a165-170 kDa member of the PDGFR (or class III) subfamily, Tyrosine Kinasefamily, Protein Kinase superfamily of molecules. Mature mouse PDGF R alpha is a 1065 amino acid (aa) type I transmembrane glycoproteinthat contains five Ig-like domains within a 504 aa extracellular domain. It is expressed on a wide variety of celltypes, both fetal and adult. In thefetus, PDGF R alpha is found on NG2+oligodendroglial precursors, mesenchyme of both the sclerotome and dermatome(but not myotome), mesenchyme of the lung, hepatoblasts, Leydig cells, andneural crest cells that target the branchial arches. In the adult, PDGF R alpha has been found constitutively or induciblyon hepatocytes, vascular and visceral smooth muscle, Leydig cells, retinalastrocytes, fibroblasts, monocytes, hepatic stellate cells, CD44+ adipocyteprecursors, endothelial cells, osteoblasts, and Ptch+ Gli+ pericryptal colonicsubepithelial fibroblasts. On the cellsurface, PDGF R alpha is a monomer. Upon binding to a dimeric ligand, PDGF R alpha either homodimerizes, or heterodimerizeswith PDGF R beta. The PDGF R alpha homodimer binds PDGF-AA, -AB, -BB, and -CC, while the alpha beta -heterodimer binds PDGF-AB, -BB, and -CC. Over aa 25-524, mouse PDGF R alpha shares 93% and 85% aa sequence identitywith rat and human PDGF R alpha,respectively. Over the same aa sequence,mouse PDGF R alpha shares only 33% aasequence identity with mouse PDGF R beta. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of PDGF R alpha in Mouse Bone Marrow by Flow Cytometry. Mouse bone marrow was stained with Rat Anti-Mouse CD45 APC‑conjugated Monoclonal Antibody (Catalog # FAB114A) and either (A) Rat Anti-Mouse PDGF R alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB9215G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins. | |
Detection of PDGF R alpha in Mouse Compact Bone Tissue by Flow Cytometry. Mouse compact bone tissue was stained with Rat Anti-Mouse CD45 APC‑conjugated Monoclonal Antibody (Catalog # FAB114A) and either (A) Rat Anti-Mouse PDGF R alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB9215G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins. |
