| 货号 | FAB530F-025 |
| 别名 | CD124 antigen; CD124; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL4R; IL-4RA; IL4RACD124; IL-4R-alpha; interleukin 4 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha | 全称 | Interleukin 4 Receptor alpha |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Detects mouse IL-4 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) IL-4 R, rhIL-9 R, rhIL-13 R alpha 1, and recombinant mouse IL-13 R alpha 2 is observed. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3566 (Human); 16190 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Multiplex immunoassay characterization and species comparison of inflammation in acute and non-acute ischemic infarcts in human and mouse brain tissue | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse IL-4 R alpha |
| 生物活性 | Mouse |
| 标记 | Fluorescein |
| 背景 | Interleukin 4 (IL-4) is a pleiotropic cytokine produced primarily by activated T cells, mast cells and basophils. The diverse biological effects of IL-4 on a variety of cell types are mediated by the binding of IL-4 to specific cell surface receptors. As is the case with many other cytokines, the functional high-affinity receptor for IL-4 is a complex consisting of a ligand binding subunit ( alpha chain) and a second subunit ( beta chain) that can modulate the ligand binding affinity of the receptor complex. It has been shown that in certain cell types, the gamma chain of the IL-2 receptor is a functional component ( beta chain) of the IL-4 receptor complex. cDNA clones for the ligand binding chain (IL-4 R alpha ) of both the mouse and human high affinity IL-4 receptors have been isolated. The human or mouse IL-4R alpha is an approximately 140 kDa transmembrane protein containing an extracellular domain, a transmembrane domain, and a large cytoplasmic domain that is essential for IL-4 signal transduction. In addition to the cDNA clone encoding the full-length transmembrane protein, a second cDNA clone that arises from alternate splicing and that encodes a soluble secreted form of IL-4 R alpha has been isolated from mouse cells, but not yet from human sources. A naturally-occurring soluble form of the IL-4 R alpha has also been identified in mouse biological fluids and murine cell culture supernatants. Native or recombinant murine soluble IL-4 R alpha, as well as recombinant human soluble IL-4 R alpha, can bind IL-4 with the same affinity as the membrane bound IL-4 R alpha. Soluble IL-4 R alpha is a competitive inhibitor of IL-4 and has been shown to neutralize effectively many IL-4-mediated responses both in vivoandin vitro. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of IL‑4 R alpha in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse IL‑4 R alpha Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB530F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins. |
