| 货号 | FAB5218F |
| 别名 | BA2; BA-2/p24 antigen; BTCC-1; CD9 antigen; CD9 molecule; CD9; Cell growth-inhibiting gene 2 protein; DRAP-27; Leukocyte antigen MIC3; MIC3; motility related protein-1,5H9 antigen; Motility-related protein; MRP-1FLJ99568; p24; tetraspanin-29; TSPAN29; TSPAN-29; TSPAN29MIC3CD9 antigen (p24) |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(10 µL/106cells) |
| 目标/特异性 | Stains mouse CD9-transfected cells in flow cytometry, but not irrelevant transfectants. |
| 使用方法 | Flow Cytometry: 10 µL/106cells |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 928 (Human); 12527 (Mouse) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | NS0 mouse myeloma cell line transfected with mouse CD9 Pro2-Val226 Accession # P40240 |
| 生物活性 | Mouse |
| 标记 | Fluorescein |
| 背景 | CD9 is a 22-25 kDa member of the tetraspanin (TM4SF) superfamily of molecules. Although typology of this family shows a cytoplasmic N- and C-terminus, tetraspanins should not be confused with the MS4A (Membrane-spanning 4 domain) family of proteins. To date, there are more than 30 human tetraspanins, presumably all of which participate in the creation of tetraspanin webs (or multimolecular microdomains) that traverse the cell membrane. As with other superfamily members, CD9 forms homodimers and associates with essentially five classes of molecules: Integrins ( alpha IIb beta 3, alpha 6 beta 4, alpha 3-6 beta 1), IgSF members (ICAM-1, VCAM-1, IgSF8/EWI-2, CD9P1), ectoenzymes (ADAM-12, MT1-MMP) intracellular signaling moluecules (PI-4K, PKC) and others (CD81, CD63, IL-16, pro-HBEGF). The microdomain complexes both recruit and regulate individual molecules. CD9-containing microdomains reportedly promote gamete fusion while inhibiting monocyte fusion. Cells known to express CD9 are many and varied, and include oocytes, regulatory B cells (in mouse, not human), endothelial cells, platelets, monocytes, keratinocytes, mast cells, and myoblasts. The extracellular domain(s) or mouse CD9 share 80% and 88% amino acid sequence identity with human and rat CD9, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of CD9 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CD9 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB5218F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins. |
