Mouse CD25/IL-2 R alpha Allophycocyanin MAb (Clone PC61.5.3) (25 Tests)
货号：
FAB9164A-025 基本售价：
1685.4 元 规格：
25 Tests
产品信息
概述| 货号 | FAB9164A-025 |
| 别名 | CD25 antigen; CD25; IDDM10; IL-2 R alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2R; IL2RA; IL-2-RA; IL2-RA; interleukin 2 receptor, alpha; interleukin-2 receptor subunit alpha; p55; TAC antigen; TCGFR |
全称 | Interleukin 2 Receptor alpha | | 应用 | Flow Cytometry(10 µL/106cells)
|
| 目标/特异性 | Detects mouse CD25/IL-2 R alpha in flow cytometry. |
| 使用方法 | Flow Cytometry: 10 µL/106cells
|
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | B6.1 mouse cytotoxic T cell line |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | IL-2 receptor alpha (IL-2 R alpha ), also known as CD25, is a 55 kDa type I membrane glycoprotein that belongs to the family of cytokine receptors that utilize the common gamma chain subunit ( gamma c). IL-2 R alpha is primarily expressed on activated T cells and on regulatory T cells (Treg). The mouse IL-2 R alpha cDNA encodes a 268 amino acid (aa) precursor that includes a 21 aa signal peptide, a 215 aa extracellular domain (ECD) with two Sushi domains, a 21 aa transmembrane segment, and an 11 aa cytoplasmic domain. Within the ECD, mouse IL-2 R alpha shares 81% and 58% aa sequence identity with rat and human IL-2 R alpha, respectively. It shares approximately 15% aa sequence identity with IL-4, -7, -9, -15, and -21 receptor subunits that also complex with gamma c. IL-2 R beta (CD122) and gamma c (IL-2 R gamma /CD132) dimerize to form a constitutively expressed intermediate affinity IL-2 receptor. By itself, IL-2 R alpha binds IL-2 with low affinity. It associates with IL-2 R beta and gamma c to generate a ternary high affinity IL-2 receptor complex. A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor, rendering the T cell unresponsive to IL-2. Increased serum levels of soluble IL-2 R alpha are found in some cancers and immune disorders. IL-2 R alpha is required for Activation Induced Cell Death (AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones. IL-2 R alpha is also required for the development of CD4+CD25+ Treg which suppress autoreactive CD4+ T cells, thereby contributing to peripheral T cell homeostasis. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Acute Lymphoblastic Leukemia
- Acute Myeloid Leukemia
- B Cell Activation
- B Cell Markers
- Cancer Biomarkers
- Common gamma Chain Receptor Family
- Cytokines and Receptors in Allergy and Asthma
- Cytokines and Receptors in Tfh Cell Differentiation and Function
- Group 1 ILCs
- Group 2 ILCs
- Human CD Marker Products
- ICC / IHC Images: CD25/IL-2 R alpha
- Interactive Pathway: IL-2 Signaling and its Primary Biological Effects in Different Immune Cell Types
- Isolation, Culture, and Identification of Natural Killer Cells
- LTi Cells
- Lymphoma
- NCR+ILC3
- NCR-ILC3
- Neuroinflammation
- Preeclampsia
- Receptors in the Jak/STAT Pathway
- Regulatory T Cells (Tregs)
- T Cell Cytokine Signaling
|
参考图片 Detection of CD25/IL-2 R alpha in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rabbit Anti-Human/Mouse FoxP3 PE‑conjugated Monoclonal Antibody (Catalog # IC8214P) and either (A) Rat Anti-Mouse CD25/IL-2 R alpha APC‑conjugated Monoclonal Antibody (Catalog # FAB9164A) or (B) Rat IgG 1 Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins. |
