Mouse CD8 alpha Alexa Fluor 488 MAb (Clone 53-6.7) (25 TESTS)【科瑞奥博】

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Mouse CD8 alpha Alexa Fluor 488 MAb (Clone 53-6.7) (25 TESTS)

货号： FAB116G-025 基本售价： 1546.6 元规格： 25 Tests

产品信息

概述
货号	FAB116G-025
别名	CD8; CD8A
反应种属	Mouse
应用	Flow Cytometry(5 µL/10⁶cells) Immunocytochemistry(5 µg/mL)
目标/特异性	Detects both the alpha and alpha chains of mouse CD8 (1).
使用方法	Flow Cytometry: 5 µL/10⁶cells Immunocytochemistry: 5 µg/mL
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	925 (Human); 12525 (Mouse); 24930 (Rat)
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse thymus or spleen
生物活性	Mouse
标记	Alexa Fluor 488
背景	CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).
运输条件	Blue Ice
存放说明	4℃
参考文献	Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280. Hayakawa, K. et al. (1994) Science 263:1131. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927. Rocha, B. et al. (1992) Immunol. Today 13:449. Wang, J. and J.R. Klein (1994) Science 265:1860. Vermec, D. et al. (1992) J. Exp. Med. 176:47. Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.

参考图片

Detection of CD8 alpha in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD8 alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB116G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.

CD8 alpha in Mouse Splenocytes. CD8 alpha was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD8 alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB116G, green) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.