Mouse TER-119 Fluorescein MAb (Clone TER-119) (25 TESTS)
货号:
FAB1125F-025 基本售价:
1407.8 元 规格:
25 Tests
产品信息
概述货号 | FAB1125F-025 |
别名 | Ly76; Ly-76; TER119 |
反应种属 | Mouse |
应用 | Flow Cytometry(10 µL/106cells)
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目标/特异性 | Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, and adult peripheral blood. |
使用方法 | Flow Cytometry: 10 µL/106cells
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来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
产品组分 | |
性能供应商 | R&D Systems |
Entrez Gene IDs | 104231 (Mouse) |
纯化方式 | Protein A or G purified from hybridoma culture supernatant |
免疫原 | C57BL/6 mouse day-14 fetal liver cells |
生物活性 | Mouse |
标记 | Fluorescein |
背景 | The monoclonal antibody TER-119 is isolated from a hybridoma generated using splenocytes from a rat subcutaneously injected with day 14 BALB/c fetal liver cells (1). The TER-119 monoclonal antibody reacts with erythroid cells from the early proerythroblast to mature erythrocyte stages of development (1). The 52 kDa ligand for TER-119 is associated with glycophorin A on erythrocytes (1). TER-119 antibodies are frequently used in combination with other lineage depletion antibodies to enrich for mouse hematopoietic stem cells (2, 3). |
运输条件 | Blue Ice |
存放说明 | 4℃ |
参考文献 | - Kina, T. et al. (2000) Br. J. Haematol. 109:280.
- Ikuta, K. et al. (1990) Cell 62:863.
- Osawa, M.Y. et al., (1996) Hematopoietic Stem Cells in Weir’s Handbook of Experimental Immunology, Vol. 2, 5th Edition. Herzenberg, L.A. et al. eds. Blackwell Science, Cambridge, MA. pp. 66.1-66.5
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参考图片Detection of TER‑119 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse CD45 PE‑conjugated Monoclonal Antibody (Catalog # FAB114P) and either (A) Rat Anti-Mouse TER‑119 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1125F) or (B) Rat IgG 2B Fluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins. |