| 货号 | FAB554G-025 |
| 别名 | CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry(5 µL/106cells) Immunocytochemistry(5 µg/mL) |
| 目标/特异性 | Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells. |
| 使用方法 | Flow Cytometry: 5 µL/106cells Immunocytochemistry: 5 µg/mL |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse CTL clone V4 |
| 生物活性 | Mouse |
| 标记 | Alexa Fluor 488 |
| 背景 | CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of CD4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD4 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB554G, open histogram) or isotype control antibody (Catalog # IC013G, filled histogram). View our protocol for Staining Membrane-associated Proteins. | |
CD4 in Mouse Splenocytes. CD4 was detected and stained in immersion fixed mouse splenocytes using Rat Anti-Mouse CD4 Alexa Fluor® 488‑conjugated Monoclonal Antibody (green, Catalog # FAB554G) at 5 μg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells. |
