Mouse CD117/c-kit Phycoerythrin MAb (Clone 180627) (25 TESTS)【科瑞奥博】

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Mouse CD117/c-kit Phycoerythrin MAb (Clone 180627) (25 TESTS)

货号： FAB1356P-025 基本售价： 1120.3 元规格： 25 Tests

产品信息

概述
货号	FAB1356P-025
别名	CD117 antigen; CD117; ckit; c-kit; EC 2.7.10; EC 2.7.10.1; KIT; PBT; piebald trait; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCF R; SCFRmast/stem cell growth factor receptor; soluble KIT variant 1; Tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
全称	Stem Cell Factor Receptor
应用	Flow Cytometry
目标/特异性	Detects mouse SCF R in direct ELISAs.
使用方法	Flow Cytometry: 10 µL/10⁶cells
来源	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3815 (Human); 16590 (Mouse); 64030 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. IL-21 Reduces Immediate Hypersensitivity Reactions in Mouse Skin by Suppressing Mast Cell Activation or IgE Production. Authors: Tamagawa-Mineoka R, Kishida T, Mazda O J. Invest. Dermatol., 2011;131(7):1513-20. Species: Mouse Sample Type: Whole Cells Application: Flow Mobilized bone marrow progenitor cells serve as donors of cytoprotective genes for cardiac repair. Authors: Dai Y, Ashraf M, Zuo S, Uemura R, Dai YS, Wang Y, Haider HKh, Li T, Xu M J. Mol. Cell. Cardiol., 2007;44(3):607-17. Species: Mouse Sample Type: Whole Cells Application: Flow
纯化方式	Protein A or G purified from hybridoma culture supernatant
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse SCF R Gln25-Thr519 (Ala207Glu) Accession # P05532
生物活性	Mouse
标记	Phycoerythrin
背景	SCF R, also known as c-kit proto-oncogene protein and CD117, is a 145-160 kDa member of the type III tyrosine kinase family of proteins (1,2). It is expressed on hematopoietic and endothelial cell progenitors, mast cells, germ cells, melanocytes, trigeminal neurons, glial cells, placenta, kidney, lung, and gut. SCF R is a type I transmembrane glycoprotein that undergoes splicing during spermatogenesis. Two isoforms of 50 kDa and 30 kDa are generated that represent C-terminal intracellular functional products (3). SCF R also undergoes cleavage by select cell types, generating a 95-100 kDa soluble form (4). Over amino acids 25-519, mouse SCF R shares 73% and 87% amino acid sequence identity with human and rat SCF R, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Heldin, C-H. and J. Lennartsson (2013) Cold Spring Harb. Perspect. Biol. 5:a009100. Lennartsson, J. and L. Ronnstrand (2012) Physil. Rev. 92:1619. Zhang, L. et al. (2013) BMC Dev. Biol. 13:38. Broudy, V.C. et al. (2001) Cytokine 15:188.

参考图片

Detection of CD117/c-kit in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE‑conjugated Monoclonal Antibody (Catalog # FAB1356P) and Rat Anti-Mouse Sca‑1/Ly6 APC‑conjugated Monoclonal Antibody
(Catalog #FAB1226A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.