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货号： bs-1082R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1082R
- 英文名称
- GRK1
- 中文名称
- G蛋白偶合受体激酶1抗体
- 别 名
- G-protein coupled receptor kinase 1; GRK1; GPRK1; RK; Grk1; Rhok; RHODOPSIN KINASE; RK_HUMAN.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 免疫学 神经生物学 信号转导 激酶和磷酸酶 细胞膜受体 G蛋白偶联受体 G蛋白信号
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Cow,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 62kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human GRK1:201-300/563
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq]
Function:
Phosphorylates rhodopsin thereby initiating its deactivation. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
Subcellular Location:
Membrane.
Tissue Specificity:
Retina and pineal gland.
Post-translational modifications:
Autophosphorylated.
Farnesylation is required for full activity.
DISEASE:
Defects in GRK1 are a cause of congenital stationary night blindness Oguchi type 2 (CSNBO2) [MIM:613411]. It is non-progressive retinal disorder characterized by impaired night vision, often associated with nystagmus and myopia. Congenital stationary night blindness Oguchi type is associated with fundus discoloration and abnormally slow dark adaptation.
Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain.
SWISS:
Q15835
Gene ID:
6011
Database links:Entrez Gene: 6011Human
Entrez Gene: 24013Mouse
Omim: 180381Human
SwissProt: Q15835Human
SwissProt: Q9WVL4Mouse
Unigene: 103501Human
Unigene: 721727Human
Unigene: 257501Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
G蛋白偶联受体激酶1(GRK1)是催化激动剂诱导的GPCR磷酸化以及启动GPCR脱敏的关键激酶,主要用于GRK细胞膜转位和对GRK功能的影响.
- 产品图片
Sample: Raji Cell Lysate at 40 ug
Primary: Anti-GRK1 (bs-1082R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size: 62 kD
Observed band size: 62 kD
Sample:
K562 Cell (Human) Lysate at 30 ug
Primary: Anti-GRK1 (bs-1082R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 65 kD
Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GRK1 Polyclonal Antibody, Unconjugated(bs-1082R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GRK1 Polyclonal Antibody, Unconjugated(bs-1082R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining