| 货号 | 15032 |
| 同种亚型 | IgG |
| 反应种属 | Mouse |
| 来源宿主 | Goat |
| 使用方法 | Applications: Immunofluorescence: 1:100 to 1:250 dilution Plate-based assays: 1:800 to 1:1,000 dilution To ensure a maximum in photostability of Chromeo 494 conjugates under all experimental conditions, we recommend the use of Mowiol or TDE as mounting medium. Vectashield or MAXfluor Mounting medium might negatively influence the photostability of Chromeo 494. Chromeo 494 Goat anti-Mouse IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using Leicas STimulatedEmissionDepletion(STED) microscopes. STED enables resolution of 50-70 nm.  Absorption and emission spectra of Chromeo 494 Dye.  Active Motifs primary antibodies and fluorescent secondary antibodies in STED microscopy. HeLa cells were stained with alpha Tubulin mouse monoclonal antibody (Clone 5-B-1-2, Cat. No. 39527)andChromeo 494 Goat anti-Mouse IgG. Histone H3 was stained with Histone H3 trimethyl Lys4 rabbit polyclonal antibody (Cat. No. 39159) and the ATTO 647N (STED/GSD) Goat anti-Rabbit IgG (Cat. No. 15048) secondary antibody. This STED (STimulatedEmissionDepletion) image is courtesy of Leica Microsystems, Germany. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 运输条件 | Room Temp |
| 存放说明 | The conjugated antibody should be stored at 4°C protected from light. |
| 外观 | Contents: 1 mg (Catalog No. 15032) or 0.1 mg (Catalog No. 15052) of Chromeo™ 494 conjugated Goat anti-Mouse IgG (H+L). The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM sod |
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Absorption and emission spectra of Chromeo 494 Dye. | |
Active Motifs primary antibodies and fluorescent secondary antibodies in STED microscopy. HeLa cells were stained with alpha Tubulin mouse monoclonal antibody (Clone 5-B-1-2, Cat. No. 39527) and Chromeo 494 Goat anti-Mouse IgG. Histone H3 was stained with Histone H3 trimethyl Lys4 rabbit polyclonal antibody (Cat. No. 39159) and the ATTO 647N (STED/GSD) Goat anti-Rabbit IgG (Cat. No. 15048) secondary antibody. This STED (STimulatedEmissionDepletion) image is courtesy of Leica Microsystems, Germany. |
