| 货号 | 15030 |
| 同种亚型 | IgG |
| 反应种属 | Mouse |
| 来源宿主 | Goat |
| 使用方法 | Applications: Immunofluorescence: 1:1,000 to 1:2,000 dilution Plate-based assays: 1:800 to 1:1,000 dilution Chromeo 505 Goat anti-Mouse IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using Leicas STimulatedEmissionDepletion(STED) microscopes. It has also been tested and verified by Leica for use in GroundStateDepletion with IndividualMolecule return (GSDIM) using the SR GSD microscope. STED enables resolution of 50-70 nm, while GSDIM can mathematically re-construct images with resolution as low as 20 nm.  Absorption and emission spectra of Chromeo 505 Dye.  Chromeo 505 staining in HeLa cells. HeLa cells were stained with alpha Tubulin mouse mAb (Clone 5-B-1-2; Cat. No. 39527)andChromeo 505 Goat anti-Mouse IgG.  Multi-color staining of Phospho H2AX and tubulin using Chromeo 488 and Chromeo 494 secondary antibodies in etoposide-treated HeLa cells. HeLa cells were treated with 100 µM etoposide for 6 hours prior to fixation with methanol. The histone-variant H2AX was stained with Histone H2AX phospho Ser139 rabbit pAb (Cat. No. 39117)andChromeo 642 Goat anti-Rabbit IgG (Cat. No. 15044), while tubulin was visualized using alpha Tubulin mouse mAb (Clone 5-B-1-2, Cat. No. 39527)andChromeo 505 Goat anti-Mouse IgG. The nuclei were counter-stained with DAPI and then the three separate images were merged. |
| 供应商 | Active Motif |
| 纯化方式 | Affinity Purified |
| 运输条件 | Room Temp |
| 存放说明 | The conjugated antibody should be stored at 4°C protected from light. |
| 外观 | Contents: 1 mg (Catalog No. 15030) or 0.1 mg (Catalog No. 15050) of Chromeo™ 505 conjugated Goat anti-Mouse IgG (H+L). The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM sod |
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Absorption and emission spectra of Chromeo 505 Dye. | |
Chromeo 505 staining in HeLa cells. HeLa cells were stained with alpha Tubulin mouse mAb (Clone 5-B-1-2; Cat. No. 39527) and Chromeo 505 Goat anti-Mouse IgG. | |
Multi-color staining of Phospho H2AX and tubulin using Chromeo 488 and Chromeo 494 secondary antibodies in etoposide-treated HeLa cells. HeLa cells were treated with 100 µM etoposide for 6 hours prior to fixation with methanol. The histone-variant H2AX was stained with Histone H2AX phospho Ser139 rabbit pAb (Cat. No. 39117) and Chromeo 642 Goat anti-Rabbit IgG (Cat. No. 15044), while tubulin was visualized using alpha Tubulin mouse mAb (Clone 5-B-1-2, Cat. No. 39527) and Chromeo 505 Goat anti-Mouse IgG. The nuclei were counter-stained with DAPI and then the three separate images were merged. |
