| 货号 | F0117 |
| 别名 | AGM1; DKFZp686I15196; DKFZp686I15212; FLJ00385; immunoglobulin heavy constant mu; MGC104996; MGC52291; MU; VH | 全称 | Immunoglobulin M |
| 同种亚型 | IgM |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Mouse IgM specific IgG was purified by goat IgG affinity chromatography and then adsorbed to eliminate human cross reactivity. The IgG fraction was digested with pepsin to generate F(ab)2 fragments which have a reduced ability to interact with Fc receptors expressed on a variety of cells. |
| 使用方法 | Flow Cytometry: Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of the IgM class. The recommended concentration is 10 μL/106cells. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse IgM |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of MouseIgM Primary Antibody by Flow Cytometry BG01V human embryonic stem cells were stained with MouseAnti-Human TRA-1-81 Monoclonal Antibody (Catalog # MAB8495,filled histogram) or mouse IgM isotype control antibody (open histogram),followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog #F0117) at 10 μL/106cells. |
