| 货号 | F0101B |
| 别名 | immunoglobulin heavy constant gamma 1 (G1m marker) | 全称 | Immunoglobulin G |
| 同种亚型 | IgG |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Detects mouse IgG primary antibodies in immunofluorescent assays. After purification by affinity chromatography, this antibody was adsorbed to eliminate human cross-reactivity and the IgG fraction was further digested with pepsin to generate F(ab)2 fragments which have reduced interactions with cell surface Fc receptors |
| 使用方法 | Flow Cytometry: Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, where the primary antibody does not have a fluorescent reporter, is of mouse origin, and is of IgG class. The recommended concentration is 10 μL/106cells. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Attenuated, oncolytic, but not wild-type measles virus infection has pleiotropic effects on human neutrophil function. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse IgG heavy and light chains |
| 生物活性 | Mouse |
| 标记 | Allophycocyanin |
| 背景 | R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of MouseIgG Primary Antibody by Flow Cytometry MCF-7 human breast cancer cell line was stained with MouseAnti-Human Calreticulin-2 Monoclonal Antibody (Catalog # MAB2927,filled histogram) or isotype control antibody (Catalog # MAB0041),open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgGSecondary Antibody (Catalog # F0101B) at 10 μL/106 cells. To facilitate intracellular staining, cellswere fixed with paraformaldehyde and permeabilized with saponin. |
