| 货号 | F0116 |
| 别名 | AGM1; DKFZp686I15196; DKFZp686I15212; FLJ00385; immunoglobulin heavy constant mu; MGC104996; MGC52291; MU; VH | 全称 | Immunoglobulin M |
| 同种亚型 | IgM |
| 反应种属 | Mouse |
| 应用 | Flow Cytometry |
| 目标/特异性 | Mouse IgM specific IgG was purified by goat IgG affinity chromatography and then adsorbed to eliminate human cross reactivity. The IgG fraction was digested with pepsin to generate F(ab)2 fragments which have a reduced ability to interact with Fc receptors expressed on a variety of cells. |
| 使用方法 | Flow Cytometry: Designed for use as a secondary developing reagent in immunofluorescent assays, such as flow cytometry, wherethe primary antibody does not have a fluorescent reporter, is of mouse origin, and is of the IgM class. Therecommended concentration is 10 μL/106cells. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse IgM |
| 生物活性 | Mouse |
| 标记 | Phycoerythrin |
| 背景 | R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of MouseIgM Primary Antibody by Flow Cytometry HeLa human cervical epithelial carcinoma cell line wasstained with Mouse Anti-Human N-Cadherin Monoclonal Antibody (Catalog #MAB13882,filled histogram) or isotype control antibody mouse IgM (open histogram),followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody(Catalog # F0116) at 10 μL/106cells. |
