Anti-mouse IgG (H+L)F(ab)2 Fragment (Alexa Fluor® 647 Conjugate)【科瑞奥博】

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Anti-mouse IgG (H+L)F(ab)2 Fragment (Alexa Fluor® 647 Conjugate)

货号： 4410S 基本售价： 1798.0 元规格： 250ul

产品信息

概述
货号	4410S
描述	Anti-Mouse IgG (H+L) F(ab)2 Fragment was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab)2 fragment results in less non-specific binding to cells through Fc receptors.
反应种属	mouse
来源宿主	Goat
应用	IF-IC/F/HCA
使用方法	IF-IC ()

性能
供应商	CST
标记	Alexa Fluor 647
背景	Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

参考图片

Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb #2966 detected with Anti-Mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) (green) compared to a nonspecific negative control antibody (red).流式细胞术分析未处理的Jurkat细胞，所用抗体为Akt (5G3) Mouse mAb #2966，检测抗体为Anti-Mouse IgG (H+L), F(ab’)2Fragment (Alexa Fluor ® 647 Conjugate) (绿色) ，与非特异性阴性对照抗体（红色）作对比。

High content analysis of C2C12 cells exposed to varying concentrations of U0126 #9903 for 2 hours. With increasing concentrations of U0126, a significant decrease (~20 fold) in phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) signal as compared to the untreated control was observed. When using Phospho-p44/42 MAPK (Erk1/2) protein as a measurement, the IC50 of this compound was 2.5 μM. Data was generated on the Acumen® HCS platform using Anti-Mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate).高通量分析C2C12细胞，该细胞暴露于LY294002 #9901 2个小时。与未处理的对照组相比较，随着LY294002 浓度的增加phospho-S6 Ribosomal protein (Ser202/204)信号显著降低（约20倍）。当采用 Phospho-p44/42 MAPK (Erk1/2)检测时，IC50为2.5mM。数据时以Acumen ® HCS为平台生成的，所用抗体为Anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 647 Conjugate)。

Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb #4545 detected with Anti-Mouse IgG (H+L), F(ab)2 Fragment (Alexa Fluor® 647 Conjugate) (blue). Red = propidium iodide (fluorescent DNA dye).激光共聚焦免疫荧光分析HeLa细胞，所用抗体为g Pan-Keratin (C11) Mouse mAb #4545检测抗体为anti-Mouse IgG (H+L), F(ab’)2 Fragment (Alexa Fluor ® 647 Conjugate) (蓝色)。红色为propidium iodide，荧光DNA染料。