| 货号 | 8911SC |
| 供应商 | CST |
| 背景 | Leukemia Inhibitory Factor (LIF) is a 20 kDa pleiotrophic factor belonging to the IL-6 superfamily of cytokines (1). LIF is expressed in a number of tissues and cell types. The LIF receptor is a heterodimer comprised of LIF-R (gp190) and gp130, a common signal transducer for IL-6-type cytokines (1). Depending on cell type and context, LIF/LIF-R can activate Erk, PI3K, and Jak1/Stat1/3 pathways (1,2). LIF has a diverse array of biological activities. Murine embryonic stem cells are dependent on LIF for pluripotency and self-renewal in vitro (1). Exercise- induced LIF secretion in muscle induces myoblast proliferation, suggesting that LIF may play a role in exercise-induced muscle hypertrophy (2). LIF also negatively regulates Th2 and Th17 cell differentiation (3,4). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hLIF. All lots are greater than 98% pure. |
The purity of recombinant hLIF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hLIF and staining overnight with Coomassie Blue.重组hLIF的纯度通过SDS-PAGE进行测定。用6 µg还原的(+)和非还原的(-)重组hLIF进行SDS-PAGE，考马斯亮蓝染色过夜。 | |
The proliferation of TF-1 cells treated with increasing concentrations of hLIF was assessed. After 72 hr treatment with hLIF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.不断增加hLIF的浓度来测定TF-1细胞的增殖能力。用hLIF作用72小时后，细胞中加入四唑盐继续培养，并测定OD450-OD650。 | |
Western blot analysis of extracts from TF-1 cells untreated or treated with hLIF for 10 min, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).Western blot分析TF-1细胞提取物，未处理或用hLIF处理10分钟，使用的抗体是：Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145（上图）、Stat3 Antibody #9132（下图）。 |
