| 货号 | 5414LC |
| 供应商 | CST |
| 背景 | FGF basic (FGF2) is producted in both embryonic and adult cell types, and contributes to the pathogenesis of various diseases, including cancer and atherosclerosis (1). FGF basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF basic has a high affinity for heparan sulfate (1,2) and binding is a step in the FGF basic activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/AKT pathways (1). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mFGF basic. All lots are greater than 98% pure. |
The purity of recombinant mFGF basic was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mFGF basic and staining overnight with Coomassie Blue.重组mFGF basic 的纯度用SDS-PAGE验证，6 µg 还原（+）和未还原（-）的重组mFGF basic 电泳后考马斯蓝染色过夜。 | |
The proliferation of NIH/3T3 cells treated with increasing concentrations of mFGF basic was assessed. After 24 hr treatment, cells were labeled with BrdU for 4 hrs. BrdU incorporation was determined by ELISA and the OD450-OD690 was determined.检验逐渐增长浓度的mFGF basic对NIH/3T3细胞增殖的影响。处理24小时后，BrdU处理4小时。BrdU掺入通过ELISA和OD450-OD690检测。 | |
Western blot analysis of extracts from NIH/3T3 cells, untreated or treated with mFGF basic for 10 min, using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb #4370 (upper) and p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (lower).mFGF basic未处理或处理10分钟后的NIH/3T3 细胞提取物进行western blot实验，使用Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® 兔单抗#4370 (上) and p44/42 MAPK (Erk1/2) (137F5) 兔单抗 #4695 (下)检测. |
