| 货号 | 5224SC |
| 供应商 | CST |
| 背景 | IL-22, a member of the IL-10 family (1,2), is expressed by Th17 CD4+ T cells, activated T cells, Th1 cells and NK cells (3). Expression of IL-22 in combination with a select group of cytokines defines a subset of Th cells(4). IL-22 induces proinflammatory responses, drives production of antimicrobial peptides, and is involved in tissue-repair and wound-healing responses (1). The IL-22 receptor is a heterodimer of IL-22R1 and IL-10R2 (5). Expression of IL-22R is restricted to tissue-resident cells, particularly those of epithelial origin, whereas the IL-10R2 chain is expressed in many more cell types. IL-22 induces phosphorylation of Jak1 and Tyk2, leading to activation of Stat3 and, to a lesser extent, Stat1 and Stat5 (1). IL-22 responses can involve activation of the MEK-ERK-RSK, JNK-SAPK, and p38 pathways (1). Elevated levels of IL-22 have been associated with Crohns disease and rheumatoid arthritis. IL-22 plays an essential role in host response to the pulmonary pathogen Klebsiella pneumoniae (6). |
| 存放说明 | 4C |
| 纯度 | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant mIL-22. All lots are greater than 98% pure. |
The purity of recombinant mIL-22 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant mIL-22 and staining overnight with Coomassie Blue.重组mIL-22蛋白的纯度通过SDS-PAGE 来确定。6 µg 经还原(+)和未经过还原(-) 的重组mIL-22蛋白跑SDS胶并用考马斯亮蓝染色过夜。 | |
The production of IL-10 by COLO 205 cells cultured with increasing concentrations of mIL-22 was assessed. Media from cells incubated with mIL-22 for 48 hours was collected and assayed for IL-10 by ELISA and the OD450-OD650 was determined.用浓度递增的mIL-22 诱导COLO 205细胞产生IL-10能力的实验。用mIL-22孵育细胞48小时后取上清，通过ELISA 和OD450-OD650 测定IL-10 的量。 | |
Western blot analysis of extracts from HT-29 cells, untreated or treated with mIL-22 for 15 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).Western免疫印迹。用 Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (上图)和 Stat3 Antibody #9132 (下图) 研究未经处理的和经mIL-22 处理15分钟的HT-29 细胞的细胞提取液。 |
