| 货号 | 15922-25mg |
| 描述 | BCECF AM ester is a widely-used fluorescent indicator for estimating intracellular pH.1,2 This membrane-permeant compound is modified by intracellular esterases to produce BCECF, a polar fluorescein derivative that is retained by cells. Intracellular BCECF is stable and has an efflux half-life of greater than two hours.3 Intracellular pH measurements are made by ratio imaging, which involves determining the pH-dependent ratio of emission intensity (detected at 535 nm) when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm.2,4 This approach is amenable to either spectrofluorometry or flow cytometry.2,5 BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.6 |
| 别名 | BCECF Acetoxymethyl ester; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Rink, T.J.,Tsien, R.Y. and Pozzan, T. Cytoplasmic pH and free Mg2+ in lymphocytes. Journal of Cell Biology 95(1), 189-196 (1982). 2.Ozkan, P. and Mutharasan, R. A rapid method for measuring intracellular pH using BCECF-AM. Biochimica et Biophysica Acta 1572(1), 143-148 (2002). 3.Dive, C.,Cox, H.,Watson, J.V., et al. Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterases. Mol.Cell Probe 2(2), 131-145 (1988). 4.OConnor, N. and Silver, R.B. Ratio imaging: Practical considerations for measuring intracellular Ca2+ and pH in living cells. Methods Cell Biol. 81, 415-433 (2007). 5.Chow, S.,Hedley, D. and Tannock, I. Flow cytometric calibration of intracellular pH measurements in viable cells using mixtures of weak acids and bases. Cytometry 24, 360-367 (1996). 6.Balkay, L.,Márián, T.,Emri, M., et al. Flow cytometric determination of intracellular free potassium concentration. Cytometry 28, 42-49 (1997). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥95% |
| 计算分子量 | 880.7 |
| 分子式 | C42H40O21 |
| CAS号 | 117464-70-7 |
| 稳定性 | ≥ 2 years |
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