| 货号 | 520111-480Solid |
| 描述 | Leukotriene B4(LTB4) is synthesized from arachidonic acid by the combined action of 5-lipoxygenase and LTA4hydrolase.1LTB4 has long been recognized as a potent mediator of inflammation. It stimulates a number of leukocyte functions, including aggregation, stimulation of ion fluxes, enhancement of lysosomal enzyme release, superoxide anion production, chemotaxis, and chemokinesis.2,3 In subnanomolar ranges (3.9 x 10−10 M), LTB4 causes chemotaxis and chemokinesis in human PMNLs.2 At higher concentrations, (1.0 x 10−7 M), LTB4 leads to neutrophil aggregation and degranulation as well as superoxide anion production.2,4 Plasma levels of LTB4 increase from <100 pg/ml to >100 ng/ml following leukocyte stimulation.5,6,7LTB4 is metabolized in leukocytes and hepatocytes to less active 20-hydroxy and 20-carboxy LTB4 by NADPH-dependent cytochrome P450 enzymes followed by β-oxidation at the ω-end to ω-carboxy dinor LTB4 and ω-carboxy tetranor LTB3.3,8,9,10LTB4 is not excreted in the urine.11 |
| 别名 | LTB4 EIA Kit; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Samuelsson, B., and Funk, C.D. Enzymes involved in the biosynthesis of leukotriene B4. The Journal of Biological Chemisty 264, 19469-19472 (1989). 2.Ford-Hutchinson, A.W. Leukotriene B4 in inflammation. Critical Reviews in Immunology 10, 1-12 (1990). 3.Mayatepek, E., and Hoffmann, G.F. Leukotrienes: Biosynthesis, metabolism, and pathophysiologic significance. Pediatric Research 37, 1-9 (1995). 4.Fretland, D.J.,Widomski, D.L.,Anglin, C.P., et al. 6-trans-Leukotriene B4 is a neutrophil chemotoxin in the guinea pig dermis. Journal of Leukocyte Biology 49, 283-288 (1991). 5.Surette, M.E.,Odeimat, A.,Palmantier, R., et al. Reverse-phase high-performance liquid chromatography analysis of arachidonic acid metabolites in plasma after stimulation of whole blood ex vivo. Analytical Biochemistry 216, 392-400 (1994). 6.Shindo, K.,Fukumura, M., and Miyakawa, K. Leukotriene B4 levels in the arterial blood of asthmatic patients and the effects of prednisolone. European Respiratory Journal 8, 605-610 (1995). 7.Doyle, M.J.,Eichhold, T.H.,Hynd, B.A., et al. Determination of leukotriene B4 in human plasma by gas chromatography using a mass selective detector and a stable isotope labelled internal standard. Effect of NE-11740 on arachidonic acid metabolism. Journal of Pharmaceutical & Biomedical Analysis 8, 137-142 (1990). 8.Hammarström, S.,Örning, L., and Bernström, K. Metabolism of leukotrienes. Molecular and Cellular Biochemistry 69, 7-16 (1985). 9.Harper, T.W.,Garrity, M.J., and Murphy, R.C. Metabolism of leukotriene B4 in isolated rat hepatocytes. Identification of a novel 18-carboxy-19,20-dinor leukotriene B4 metabolite. The Journal of Biological Chemisty 261, 5414-5418 (1986). 10.Hansson, G.,Lindgren, J.Å.,Dahlén, S.E., et al. Identification and biological activity of novel w -oxidized metabolites of leukotriene B4 from human leukocytes. FEBS Letters 130, 107-112 (1981). 11.Serafin, W.E.,Oates, J.A., and Hubbard, W.C. Metabolism of leukotriene B4 in the monkey. Identification of the principal nonvolatile metabolite in the urine. Prostaglandins 27, 899-911 (1984). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 1 year |
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