| 货号 | 581021-480Solid |
| 描述 | The Cayman Chemical cGMP Assay is a competitive ELISA that can be used for quantification of cGMP directly obtained from cell lysates, tissue homogenates, plasma, or urine. The assay has a range from 0.23-30 pmol/ml and a sensitivity (80% B/B0) of approximately 1 pmol/ml. Since the antibody used in this assay was prepared against a cGMP-carrier protein conjugate, antibody binding is increased if an acetyl group is present on the 2 hydroxyl group of the cGMP. The optional acetylation procedure for both samples and standards increases the sensitivity of the assay approximately 10 fold. A protocol for acetylating both the standards and samples prior to performing the assay is provided. Basal levels of cGMP in cell lysates can often be measured without acetylation, but results will depend on the type and number of cells being utilized. Platelets produce approximately 1.5-2.5 pmol cGMP/109 platelets under basal conditions.1,2,3 Cells such as NG108-15 cells and monocytes produce considerably more cGMP than platelets (approximately 0.1-1 pmol/106cells).4,5 |
| 别名 | cGMP EIA Kit;Guanosine 3,5-cyclic mononucleotide;Guanosine 3,5-cyclic monophosphate;Monosodium-GMP; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Chen, L.,Salafranca, M.N., and Mehta, J.L. Cyclooxygenase inhibition decreases nitric oxide synthase activity in human platelets. American Journal of Physiology 42, H1854-H1859 (1997). 2.Miller, O.V., and Gorman, R.R. Modulation of platelet cyclic nucleotide content by PGE1 and the prostaglandin endoperoxide PGG2. Journal of Cyclic Nucleotide Research 2, 79-87 (1976). 3.Wu, C.C.,Ko, F.N.,Kuo, S.C., et al. YC-1 inhibited human platelet aggregation through NO-independent activation of soluble guanylate cyclase. British Journal of Pharmacology 116, 1973-1978 (1995). 4.Arima, T.,Ohshima, Y.,Mizuno, T., et al. Cyclic GMP elevation by 5-hydroxytryptamine is due to nitric oxide derived from endogenous nitrosothiol in NG108-15 cells. Biochemical and Biophysical Research Communications 227, 473-478 (1996). 5.Kolb, J.P.,Paul-Eugene, N.,Damais, C., et al. Interleukin-4 stimulates cGMP production by IFN-γ-activated human monocytes. Involvement of the nitric oxide synthase pathway. The Journal of Biological Chemisty 269, 9811-9816 (1994). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 1 year |
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