| 货号 | 516360-480Well |
| 描述 | The isoprostanes are a family of eicosanoids of non-enzymatic origin produced by the random oxidation of tissue phospholipids by oxygen radicals. Isoprostanes appear as artifacts in tissue and plasma samples which have undergone oxidative degradation during prolonged or improper storage. They also appear in the plasma and urine under normal conditions and are elevated by oxidative stress. At least one of the isoprostanes, 8-isoprostane (8-epiPGF2α), has been shown to have biological activity. It is a potent pulmonary and renal vasoconstrictor1 and has been implicated as a causative mediator of hepatorenal syndrome and pulmonary oxygen toxicity.2 8-Isoprostane has been proposed as a marker of antioxidant deficiency and oxidative stress and elevated levels have been found in heavy smokers.3 8-Isoprostane levels are also a relative indicator of sample integrity for lipid-containing samples such as serum, plasma, and whole cell preparations.4 Plasma from healthy volunteers contains modest amounts of 8-isoprostane (40-100 pg/ml) that increase with the age of the test subject.5 Normal human urinary levels range from 10-50 ng/mmol creatinine, which is an order of magnitude higher than many enzymatically derived eicosanoids.5,6 Cayman’s 8-Isoprostane Express ELISA is a competitive assay that can be used for quantification of 8-isoprostane in plasma, serum, urine, whole blood, tissue culture supernatants, and other sample matrices. |
| 别名 | iPF2α-III;8-Isoprostane Express EIA Kit;8-epi Prostaglandin F2α;8-iso Prostaglandin F2α; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Banerjee, M.,Kang, K.H.,Morrow, J.D., et al. Effects of a novel prostaglandin, 8-epi-PGF2α, in rabbit lung in situ. American Journal of Physiology 263, H660-H663 (1992). 2.Vacchiano, C.A., and Tempel, G.E. Role of nonenzymatically generated prostanoid, 8-iso-PGF2α, in pulmonary oxygen toxicity. Journal of Applied Physiology 77, 2912-2917 (1994). 3.Morrow, J.D.,Frei, B.,Longmire, A.W., et al. Increase in circulating products of lipid peroxidation (F2-isoprostanes) in smokers. New England Journal of Medicine 332, 1198-1203 (1995). 4.Morrow, J.D.,Hill, K.E.,Burk, R.F., et al. A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanism. Proceedings of the National Academy of Sciences of the United States of America 87, 9383-9387 (1990). 5.Wang, Z.,Ciabattoni, G.,Créminon, C., et al. Immunological characterization of urinary 8-epi-prostaglandin F2α excretion in man. Journal of Pharmacology and Experimental Therapeutics 275, 94-100 (1995). 6.Reilly, M.P.,Barry, P.,Lawson, J.A., et al. Urinary 8-epi PGF2α: An index of oxidant stress in vivo. Fibrinolysis & Proteolysis 11, 81-84 (1997). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 1 year |
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