| 货号 | 516011-480Well |
| 描述 | Prostaglandin F2α(PGF2α) is one of the five primary PGs derived enzymatically directly from the endoperoxide PGH2. It was initially discovered in seminal fluid, and to date the majority of the functional roles ascribed to it relate to fertility, pregnancy, and parturition. PGF2α is a potent luteolytic agent and is used to induce ovulation in domestic livestock. It is also a potent uterine stimulant, and is part of the cascade of myometrial stimulants which induce and sustain labor. PGF2α is rapidly metabolized to 13,14-dihydro-15-keto PGF2αin vivo, and the levels of this metabolite in both plasma and amniotic fluid are elevated during active labor in several different mammalian species. Like all of the primary PGs, PGF2α. has a very short half-life in the general circulation. The plasma concentration of PGF2α in humans is <10 pg/ml, and probably no more than 1-2 pg/ml.1PGF2α is rapidly metabolized by 15-hydroxy PG dehydrogenase and both β- and ω-oxidation systems to a variety of polar metabolites.2 In primary cultures, the metabolism of PGF2α is much more limited, and it may collect in the supernatant medium to easily measured concentrations.3 Amnionic and placental tissue PGF2α content in the range from 100-250 ng/g of tissue has been reported.4 However, this study did not address the presence of isoprostanes and reported measurements using an unvalidated RIA method. A controlled GC- or LC-MS study of tissue PG content has not been reported. Tissue PG measurements in general are difficult to perform, vary widely, and are challenging to interpret. |
| 别名 | PGF2α EIA Kit; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Schweer, H.,Kammer, J., and Seyberth, H.W. Simultaneous determination of prostanoids in plasma by gas chromatography-negative-ion chemical ionization mass spectrometry. Journal of Chromatography 338, 273-280 (1985). 2.Granström, E., and Kindahl, H. Species differences in circulating prostaglandin metabolites relevance for the assay of prostaglandin release. Biochimica et Biophysica Acta 713, 555-569 (1982). 3.Ritzi, E.M.,Boto, W.O., and Stylos, W.A. Measurement of initial prostaglandin F metabolites in medium of BALB/c 3T3 and SV3T3 mouse fibroblast cultures. Biochemical and Biophysical Research Communications 63, 179-186 (1975). 4.Reece, M.S.,McGregor, J.A.,Allen, K.G.D., et al. Prostaglandins in selected reproductive tissues in preterm and full term gestations. Prostaglandins, Leukotrienes and Essential Fatty Acids 55, 303-307 (1996). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 1 year |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |