| 货号 | 13891-25mg |
| 描述 | (S)-nitro-Blebbistatin is a more stable form of (-)-blebbistatin (Item No. 13013), which is a selective cell-permeable inhibitor of non-muscle myosin II ATPases.1,2 (-)-Blebbistatin rapidly and reversibly inhibits Mg-ATPase activity and in vitro motility of non-muscle myosin IIA and IIB for several species (IC50 = 0.5-5 µM), while poorly inhibiting smooth muscle myosin (IC50 = 80 µM).3 Through these effects, it blocks apoptosis-related bleb formation, directed cell migration, and cytokinesis in vertebrate cells. However, prolonged exposure to blue light (450-490 nm) results in degradation of blebbistatin to an inactive product via cytotoxic intermediates, which may be problematic for its use in fluorescent live cell imaging applications.4,5 The addition of a nitro group stabilizes the molecule to circumvent its degradation by prolonged blue light exposure.6 (S)-nitro-Blebbistatin has the same stereochemistry as the active (-)-blebbistatin enantiomer. |
| 别名 | S-(−)-7-Desmethyl-8-nitro Blebbistatin; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Straight, A.F.,Cheung, A.,Limouze, J., et al. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 299(5613), 1743-1747 (2003). 2.Kovács, M.,Tóth, J.,Hetényi, C., et al. Mechanism of blebbistatin inhibition of myosin II. J. Biol. Chem. 279(34), 35557-35563 (2004). 3.Limouze, J.,Straight, A.F.,Mitchison, T., et al. Specificity of blebbistatin, an inhibitor of myosin II. J. Muscle Res. Cell Motil. 25(4-5), 337-341 (2004). 4.Kolega, J. Phototoxicity and photoinactivation of blebbistatin in UV and visible light. Biochem. Biophys. Res. Commun. 320(3), 1020-1025 (2004). 5.Sakamoto, T.,Limouze, J.,Combs, C.A., et al. Blebbistatin, a myosin II inhibitor, is photoinactivated by blue light. Biochemistry 44(2), 584-588 (2005). 6.Lucas-Lopez, C.,Patterson, S.,Blum, T., et al. Absolute stereochemical assignment and fluorescence tuning of the small molecule tool, (−)-blebbistatin. European J. Org. Chem. 2005(9), 1736-1740 (2005). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 323.3 |
| 分子式 | C17H13N3O4 |
| CAS号 | 856925-75-2 |
| 稳定性 | ≥ 2 years |
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