| 货号 | 9001369-25mg |
| 描述 | Smoothened (SMO) is a GPCR-like receptor which, with Patched, mediates hedgehog signaling to regulate gene expression through the Gli transcription factors.1 20(S)-hydroxy Cholesterol (20(S)-OHC) is an oxysterol which binds SMO and activates hedgehog signaling (EC50 = 3 μM), and this activation is selective for the nat-20(S)-OHC enantiomer.2Nat-20(S)-OHC synergizes with the SMO agonist SAG, suggesting an allosteric effect.2Nat-20(S)-yne is a form of nat-20(S)-OHC with a terminal alkyne group, which can be used in linking reactions known as click chemistry. Click chemistry involves highly dependable and specific azide-alkyne bioconjugation reactions and can be used to capture or immobilize bioactive molecules.3,4Thus,nat-20(S)-yne has been conjugated with magnetic beads to demonstrate that nat-20(S)-OHC directly binds SMO.2 |
| 别名 | Click Tag™ Nat-20(S)-yne; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Ruiz-Gómez, A.,Molnar, C.,Holguín, H., et al. The cell biology of Smo signalling and its relationships with GPCRs. Biochimica et Biophysica Acta 1768(4), 901-912 (2007). 2.Nachtergaele, S.,Mydock, L.K.,Krishnan, K., et al. Oxysterols are allosteric activators of the oncoprotein Smoothened.. Nature Chemical Biology 8(2), 211-220 (2012). 3.Kho, Y.,Kim, S.C.,Jiang, C., et al. A tagging-via-substrate technology for detection and proteomics of farnesylated proteins. Proceedings of the National Academy of Sciences of the United States of America 101(34), 12479-12484 (2004). 4.Lutz, J.F., and Zarafshani, Z. Efficient construction of therapeutics, bioconjugates, biomaterials and bioactive surfaces using azide-alkyne "click" chemistry. Advanced Drug Delivery Reviews 60, 958-970 (2008). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥95% |
| 计算分子量 | 370.6 |
| 分子式 | C25H38O2 |
| CAS号 | 1397692-46-4 |
| 稳定性 | ≥ 2 years |
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