| 货号 | 14100-10mg |
| 描述 | During unfavorable environmental conditions, C. elegans larvae undergo arrest and form dauer larvae that can attach to other animals as a parasitic strategy for survival.1,2 DAF-12 is an orphan nuclear hormone receptor that regulates dauer larva diapause, reproductive development, fat metabolism, and life cycle/longevity in C. elegans.3,4,5 DAF-12 is required for entry into dauer when it is not bound to ligand by acting as a transcriptional repressor of target genes that favor reproductive development. Δ4-Dafachronic acid is a racemic version of a sterol-derived hormone that acts as a ligand of DAF-12.3,6,7,8Δ4-Dafachronic acid binds to and transactivates DAF-12, rescuing hormone deficiency in transgenic dauer larvae and promoting reproductive maturation into adults.6,9,1 The (25S) diastereomer of Δ4-dafachronic acid is significantly more potent than its (25R) counterpart (EC50s = 23 and 66 nM, respectively).7 |
| 别名 | 3-keto-4-Cholestenoic Acid; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Ogawa, A.,Streit, A.,Antebi, A., et al. A conserved endocrine mechanism controls the formation of dauer and infective larvae in nematodes. Current Biology 19, 67-71 (2009). 2.Wang, Z.,Zhou, X.E.,Motola, D.L., et al. Identification of the nuclear receptor DAF-12 as a therapeutic target in parasitic nematodes. Proceedings of the National Academy of Sciences of the United States of America 106(23), 9138-9143 (2009). 3.Martin, R.,Däbritz, F.,Entchev, E.V., et al. Stereoselective synthesis of the hormonally active (25S)-Δ7-dafachronic acid, (25S)-Δ4-dafachronic acid, (25S)-dafachronic acid, and (25S)-cholestenoic acid. Organic & Biomolecular Chemistry 6, 4293-4295 (2008). 4.Dumas, K.J.,Guo, C.,Wang, X., et al. Functional divergence of dafachronic acid pathways in the control of C. elegans development and lifespan. Dev.Biol. 340(2), 605-612 (2010). 5.Cheong, M.C.,Na, K.,Kim, H., et al. A potential biochemical mechanism underlying the influence of sterol deprivation stress on Caenorhabditis elegans longevity. J.Biol.Chem 286(9), 7248-7256 (2011). 6.Motola, D.L.,Cummins, C.L.,Rottiers, V., et al. Identification of ligands for DAF-12 that govern dauer formation and reproduction in C. elegans. Cell 124, 1209-1223 (2006). 7.Sharma, K.K.,Wang, Z.,Motola, D.L., et al. Synthesis and activity of dafachronic acid ligands for the C. elegans DAF-12 nuclear hormone receptor. Molecular Endocrinology 23(5), 640-648 (2009). 8.Beckstead, R.B. and Thummel, C.S. Indicted: Worms caught using steroids. Cell 124, 1137-1140 (2006). 9.Martin, R.,Entchev, E.V.,Kurzchalia, T.V., et al. Steroid hormones controlling the life cycle of the nematode Caenorhabditis elegans: Stereoselective synthesis and biology. Organic & Biomolecular Chemistry 8, 739-750 (2010). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥95% |
| 计算分子量 | 414.6 |
| 分子式 | C27H42O3 |
| CAS号 | 23017-97-2 |
| 稳定性 | ≥ 2 years |
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