| 货号 | 13221-5mg |
| 描述 | 3-hexanoyl-NBD-Cholesterol is a fluorescently-tagged cholesterol derivative with the hydrophilic NBD fluorophore attached to carbon 3, at the hydrophilic end of cholesterol, separated by a 6-carbon spacer. This design allows the cholesterol to properly orient in membrane bilayers while the fluorescent tag is presented outside of the bilayer. This should model the behavior of cholesterol in membranes better than the previously-used 25-NBD cholesterol, which positions NBD directly on the 25th carbon of cholesterol at the hydrophobic terminus. Fluorescently-tagged lipids have been used to study their interactions with proteins,1 their utilization by cells2 and liposomes,3 and for the development of assays for lipid metabolism.4,5 |
| 别名 | 3-C6-NBD Cholesterol; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Kumagai, K.,Yasuda, S.,Okemoto, K., et al. CERT mediates intermembrane transfer of various molecular species of ceramides. The Journal of Biological Chemisty 280(8), 6488-6495 (2005). 2.Luo, M.,Jones, S.M.,Peters-Golden, M., et al. Nuclear localization of 5-lipoxygenase as a determinant of leukotriene B4 synthetic capacity. Proceedings of the National Academy of Sciences of the United States of America 100(21), 12165-12170 (2003). 3.Moreno, M.J.,Estronca, L.M.B.B. and Vaz, W.L.C. Translocation of phospholipids and dithionite permeability in liquid-ordered and liquid-disordered membranes. Biophysical Journal 91, 873-881 (2006). 4.Loidl, A.,Claus, R.,Deigner, H.P., et al. High-precision fluorescence assay for sphingomyelinase activity of isolated enzymes and cell lysates. Journal of Lipid Research 43, 815-823 (2002). 5.Tani, M.,Okino, N.,Mitsutake, S., et al. Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. Journal of Biochemistry 125, 746-749 (1999). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 662.9 |
| 分子式 | C39H58N4O5 |
| CAS号 | 201731-19-3 |
| 稳定性 | ≥ 1 year |
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