Ac-Nle-Pro-Nle-Asp-AMC
货号：
21639-5mg 基本售价：
7350.0 元 规格：
5 mg
产品信息
概述| 货号 | 21639-5mg |
| 描述 | Ac-Nle-Pro-Nle-Asp-AMC is a substrate for proteasomes that is cleaved by the caspase-like site, also known as postglutamyl peptide hydrolase (PGPH), with specific activities of 113 and 6.6 nmol/min/mg by the 26S (rabbit muscle) and 20S (yeast) proteasomes, respectively.1 Caspase-like activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm. Ac-Nle-Pro-Nle-Asp-AMC inhibits the chymotrypsin-like activity of the proteasome allosterically when bound to the caspase-like site.1 It has been used to determine L-DOPA’s (Item No. 13248) effects on protein turnover rates.2 |
| 别名 | Ac-Nle-Pro-Nle-Asp-7-amido-4-Methylcoumarin;Ac-nLPnLD-AMC; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Kisselev, A.F.,Garcia-Calvo, M.,Overkleeft, H.S., et al. The caspase-like sites of proteasomes, their substrate specificity, new inhibitors and substrates, and allosteric interactions with the trypsin-like sites. The Journal of Biological Chemisty 278(38), 35869-35877 (2003).
2.Dunlop, R.A.,Dean, R.T., and Rodgers, K.J. The impact of specific oxidized amino acids on protein turnover in J774 cells. Biochem J. 410(1), 131-140 (2008).
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| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥98% |
| 计算分子量 | 655.7 |
| 分子式 | C33H45N5O9 |
| CAS号 | 355140-49-7 |
| 稳定性 | ≥ 2 years |
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