EP4 Receptor (rat) Reporter Assay Kit
货号：
600350-1ea 基本售价：
7200.0 元 规格：
100 tests
产品信息
概述| 货号 | 600350-1ea |
| 描述 | The EP4 receptor is one of four GPCRs that mediate the actions of prostaglandin E2(PGE2). The diverse effects of PGE2actingviaEP4 receptors point to the need to identify novel agonists and antagonists, both to further elucidate the function of this receptor subtype and for use as therapeutics for various diseases. Caymans EP4 Receptor (rat) Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing DNA constructs for rat EP4 receptor and a cAMP response element regulated Secreted Alkaline Phosphatase (SEAP) reporter (EP4 Receptor Reverse Transfection Strip Plate). Cells grown on the transfection complex will express EP4 at the cell surface. Binding of agonists to EP4 initiates a signal transduction cascade resulting in expression of SEAP which is secreted into the cell culture media. Aliquots of media are removed at time intervals, beginning at approximately six hours, and SEAP activity is measured following addition of a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is simple to use and can be easily adapted to high throughput screening for therapeutic compounds regulating the activation of EP4. PGE2 is included in the kit for use as a positive control. The kit provides sufficient reagents to measure SEAP activity at three time points using the black plates included. |
| 别名 | PGE2 Receptor 4;Prostaglandin E2 Receptor 4; |
性能| 供应商 | Cayman |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 1 year |
声明| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
