| 货号 | 15379-5mg |
| 描述 | Complex I, also known as NADH:ubiquinone oxidoreductase or NADH dehydrogenase (ubiquinone), catalyzes the transfer of electrons from NADH to ubiquinone (also known as coenzyme Q10) as part of the respiratory chain leading to ATP generation. Piericidin A is an irreversible inhibitor of mitochondrial complex I that strongly associates with ubiquinone binding sites in both mitochondrial and bacterial forms of the enzyme.1,2 First identified as an insecticidal metabolite produced by Streptomyces, piericidin A was soon found to bind and inhibit complex I at nanomolar concentrations.3,4 The inhibition of complex I by piericidin A in the presence of NADH results in the generation of reactive oxygen species.5 In plants, pieridicin A inhibits photosystem II, a water-plastoquinone oxidoreductase involved in light-dependent electron transfer.6 Piericidin A also suppresses the up-regulation of the glucose-regulated protein GRP78 in glucose-deprived, etoposide-resistant HT-29 cells, resulting in cell death (IC50 = 7.7 nM).7 |
| 别名 | AR 054;Shaoguanmycin B;SN 198E; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Hatefi, Y. Flavoproteins of the electron transport system and the site of action of amytal, rotenone, and piericidin A. Proceedings of the National Academy of Sciences of the United States of America 60(2), 733-740 (1968). 2.Melo, A.M.P.,Bandeiras, T.M. and Teixeira, M. New insights into type II NAD(P)H:quinone oxidoreductases. Microbiology and Molecular Biology Reviews 68(4), 603-616 (2004). 3.Takahashi, N.,Suzuki, A. and Tamura, S. Structure of piericidin A. Journal of the American Chemical Society 87(9), 2066-2068 (1965). 4.Horgan, D.J. and Casida, J.E. Specific binding of [14C] piericidin A in the reduced nicotinamide--adenine dinucleotide dehydrogenase segment of the mitochondrial respiratory chain. Biochemistry Journal 108(1), 153-154 (1968). 5.Fato, R.,Bergamini, C.,Bortolus, M., et al. Differential effects of mitochondrial Complex I inhibitors on production of reactive oxygen species. Biochim.Biophys.Acta. 1787(5), 384-392 (2009). 6.Ikezawa, N.,Ifuku, K.,Endo, T., et al. Inhibition of photosystem II of spinach by the respiration inhibitors piericidin A and thenoyltrifluoroacetone. Bioscience, Biotechnology, and Biochemistry 66(9), 1925-1929 (2002). 7.Hwang, J.H.,Kim, J.Y.,Cha, M.R., et al. Etoposide-resistant HT-29 human colon carcinoma cells during glucose deprivation are sensitive to piericidin A, a GRP78 down-regulator. J.Cell Physiol. 215(1), 243-250 (2008). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥95% |
| 计算分子量 | 415.6 |
| 分子式 | C25H37NO4 |
| CAS号 | 2738-64-9 |
| 稳定性 | ≥ 2 years |
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