Glutathione Peroxidase Assay Kit
货号：
703102-480Well 基本售价：
6860.0 元 规格：
480 wells
产品信息
概述| 货号 | 703102-480Well |
| 描述 | Glutathione peroxidase (GPx) catalyzes the reduction of hydroperoxides, including hydrogen peroxides, by reduced glutathione and functions to protect the cell from oxidative damage. With the exception of phospholipid-hydroperoxide GPx, a monomer, all of the GPx enzymes are tetramers of four identical subunits.1,2 Each subunit contains a selenocysteine in the active site which participates directly in the two-electron reduction of the peroxide substrate.1,2 The enzyme uses glutathione as the ultimate electron donor to regenerate the reduced form of the selenocysteine.1,2 The Cayman Chemical Glutathione Peroxidase Assay Kit measures GPx activity indirectly by a coupled reaction with glutathione reductase (GR). Oxidized glutathione (GSSG), produced upon reduction of an organic hydroperoxide by GPx, is recycled to its reduced state by GR and NADPH. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the A340 is directly proportional to the GPx activity in the sample.3 The Cayman GPx Assay Kit can be used to measure all of the glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates. |
| 别名 | GPx Assay Kit; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Ceballos-Picot, I.,Trivier, J.M.,Nicole, A., et al. Age-correlated modifications of copper-zinc superoxide dismutase and glutathione-related enzyme activities in human erythrocytes. Clinical Chemistry 38, 66-70 (1992).
2.Forstrom, J.W.,Zakowski, J.J., and Tappel, A.L. Identification of the catalytic site of rat liver glutathione peroxidase as selenocysteine. Biochemistry 17, 2639-2644 (1978).
3.Paglia, D.E., and Valentine, W.N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine 70, 158-169 (1967).
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| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 6 months |
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