| 货号 | 17926-100ug |
| 描述 | A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around a histone octamer consisting of two copies of each of the core histones, H2A, H2B, H3, and H4.1 The combination of two H2A/H2B dimers and one H3/H4 tetramer create the nucleosome core.2 Histone H3 undergoes many modifications which include acetylation, methylation, and phosphorylation that are important for regulation of gene transcription.1 Citrullination is another important post-translational modification that this catalyzed the by enzymes peptidylarginine deiminases (PAD4 and PAD2). Hypercitrullination of histones leads to the development of neutrophil extracellular traps (NETs), which are a part of the inflammatory response.3,4 Neutrophils release these NETs to trap and eradicate bacteria and fungi. Failure to clear citrullinated proteins and NET components following inflammation can result in the production of autoantibodies and anti-citrullinated protein antibodies.5 The persistence of these antibodies and citrullinated proteins is associated with a number of human diseases including rheumatoid arthritis, systemic lupus erythematosus, Alzheimer’s disease, and multiple sclerosis.6 This product contains purified Histone H3 (human recombinant) (Item No. 10263) that has been modified with PAD4 enzyme, which is removed by affinity chromatography after the reaction. |
| 别名 | Histone H3.1; |
| 应用 | Immunoreactive with anti-citrulline antibodies by Western blot and ELISA |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Bhaumik, S.R.,Smith, E. and Shilatifard, A. Covalent modifications of histones during development and disease pathogenesis. Nature Structural & Molecular Biology 14(11), 1008-1016 (2007). 2.Tanaka, Y.,Tawaramoto-Sasanuma, M.,Kawaguchi, S., et al. Expression and purification of recombinant human histones. Methods: A Companion to Methods in Enzymology 33, 3-11 (2004). 3.Baka, Z.,György, B.,Géher, P., et al. Citrullination under physiological and pathological conditions. Joint Bone Spine 79, 431-436 (2012). 4.Neeli, I. and Radic, M. Knotting the NETs: Analyzing histone modifications in neutrophil extracellular traps. Arthritis Research & Therapy 14(2), 1-2 (2012). 5.Foulquier, C.,Sebbag, M.,Clavel, C., et al. Peptidyl arginine deiminase type 2 (PAD-2) and PAD-4 but not PAD-1, PAD-3, and PAD-6 are expressed in rheumatoid arthritis synovium in close association with tissue inflammation. Arthritis and Rheumatism 56(11), 3541-3553 (2007). 6.Horibata, S.,Coonrod, S.A. and Cherrington, B.D. Role for peptidylarginine deiminase enzymes in disease and female reproduction. Journal of Reproduction and Development 58(3), 274-282 (2012). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | >95% (estimated by SDS-PAGE) |
| 稳定性 | ≥ 1 year |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |