Citrulline-specific Probe
货号：
16172-1mg 基本售价：
6230.0 元 规格：
1 mg
产品信息
概述| 货号 | 16172-1mg |
| 描述 | Protein arginine deiminases (PADs) catalyze the posttranslational modification of arginine residues on proteins to form citrulline, which plays a large role in regulating gene expression.1 Abnormally high PAD activity has been observed in a host of human diseases.1,2 Citrulline-specific probe is a highly sensitive, rhodamine phenylglyoxal-based fluorophore that specifically detects protein citrullination via a chemoselective reaction between glyoxal and citrulline.3 This chemical probe (comprised of a single isomer) is capable of reacting with any citrulline-containing protein and can be analyzed with fluorescent imaging (excitation 532 nm; emission 580 nm).3 When added at 100 µM for 30 minutes at acidic pH, this probe has a reported limit of detection of ~10 ng for citrullinated histone H3 and ~1 ng for autodeiminated PAD4.3 |
| 别名 | Rhodamine Phenylglyoxal;Rh-PG; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Jones, J.E.,Causey, C.P.,Knuckley, B., et al. Protein arginine deiminase 4 (PAD4): Current understanding and future therapeutic potential. Curr. Opin. Drug Discov. Devel. 12(5), 616-627 (2009).
2.Hill, J.A.,Southwood, S.,Sette, A., et al. Cutting edge: The conversion of arginine to citrulline allows for a high-affinity peptide interaction with the rheumatoid arthritis-associated HLA-DRB1*0401 MHC class II molecule. Journal of Immunology 171, 538-541 (2003).
3.Bicker, K.L.,Subramanian, V.,Chumanevich, A.A., et al. Seeing citrulline: Development of a phenylglyoxal-based probe to visualize protein citrullination. Journal of the American Chemical Society 134(41), 17015-17018 (2012).
|
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥90% |
| 计算分子量 | 731.8 |
| 分子式 | C39H35N7O7 • H2O |
| 稳定性 | ≥ 1 year |
声明| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
