| 货号 | NBP2-29321 |
| 反应种属 | Hu, Mu, Rt, Bv, CaSpecies Glossary |
| 目标/特异性 | The NF-kB p65(Ser529/536) inhibitory peptide also contains a protein transduction (PTD) sequence(DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable. The control peptide consists of only the PTD sequence. |
| 供应商 | Novus |
| 免疫原 | Functions as a p65 decoy through phosphorylation of the Ser529/536 sites on the peptide. |
| 背景 | NF-kB has been shown to regulate the expression of a number of genes whose products are involved in inflammation, viral replication, carcinogenesis, anti-apoptosis, invasion, and metastasis. Specific adhesion molecules, chemokines, inflammatory cytokines, and cell cycle regulatory genes are affected. Thus, agents that can suppress NF-kB activation have the potential to be treatments for inflammatory diseases and cancer. The Ser529/536 sites of p65 are phosphorylated during NF-kB activation, allowing p65 nuclear translocation. This p65 inhibitory peptide contains Ser529/536 sites that are phosphorylated during NF-kB activation, thereby blocking p65 Ser529/536 phosphorylation. The NF-kB p65(Ser529/536) Inhibitor Peptide can inhibit binding of recombinant p65 protein to the DNA in a dose dependent manner and maximum inhibition occurs at 50 uM (Figure 1). It can also inhibit TNF-induced NF-kB activation in cells (See Figure 2) |
| 存放说明 | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| 浓度 | Lyoph |
Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Inhibits TNF-induced NF-kB activation: KBM-5 cells were incubated with various concentrations of peptides for 1 hr and treated with 0.1nM TNF for 30min. Nuclear extracts were prepared and NF-kB activation was analyzed by EMSA (Please refer toTakada et al, 2004 for further details). PTD-p65-P6 suppresses TNF-induced NF-kB activation by 25% at 100 uM and completely at 150 uM, where as the control peptide, PTD or p65-P6 without PTD did not have any effect. | |
Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Effect of various p65-inhibitor peptides on binding of purified recombinant p65 protein to the DNA in vitro. Recombinant p65 protein (100 ng/sample) was incubated for 30 minutes with 50uM of various peptides in 0.025 ml and then assayed for DNA binding activity by EMSA (Please refer to Takada et al, 2004 (3) for further details). |
