| 货号 | NBP2-26505 |
| 反应种属 | Hu, Mu, Rt, Bv, Ca, MaSpecies Glossary |
| 应用 | B/N, Func-Inh, In vivo |
| 供应商 | Novus |
| 免疫原 | Functions as a p65 decoy through phosphorylation of the Ser276 site on the peptide. |
| 背景 | NF-kB has been shown to regulate the expression of a number of genes whose products are involved in inflammation, viral replication, carcinogenesis, antiapoptosis, invasion, and metastasis. Specific adhesion molecules, chemokines, inflammatory cytokines, and cell cycle regulatory genes are affected. Thus, agents that can suppress NF-kB activation have the potential to be treatments for inflammatory diseases and cancer. The Ser276 site of p65 is phosphorylated during NF-kB activation, allowing p65 nuclear translocation. This p65 inhibitory peptide contains a Ser276 site that is phosphorylated during NF-kB activation, thereby blocking p65 Ser276 phosphorylation (Bharti et al, 2003; Takada et al, 2004). The NF-kB p65 (Ser276), also known as PTD-p65-P1, inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable (Derossi et al, 1994). The control peptide consists of only the PTD sequence. The PTD-p65-P1 peptide can inhibit binding of recombinant p65 protein to the DNA in a dose dependent manner and maximum inhibition occurs at 50 uM (Figure 1). It can also inhibit TNF- induced NF-kB activation in vivo. PTD-p65-P1 suppresses TNF-induced NF-kB activation by 25% at 100 uM and completely at 150 uM (figure 2). |
| 存放说明 | Store at -20C. Avoid freeze-thaw cycles. |
| 浓度 | Lyoph |
Blocking/Neutralizing: RelA/NFkB p65 [p Ser276] Inhibitor Peptide Set [NBP2-26505] - Effect of various p65-inhibitor peptides on binding of purified recombinant p65 protein to the DNA in vitro. Recombinant p65 protein (100 ng/sample) was incubated for 30 minutes with 50uM of various peptides in 0.025 ml and then assayed for DNA binding activity by EMSA (Please refer to Takada et al, 2004 for further details). | |
Blocking/Neutralizing: RelA/NFkB p65 [p Ser276] Inhibitor Peptide Set [NBP2-26505] - PTD-p65-P1 peptide inhibits TNF-induced NF-kB activation: KBM-5 cells were incubated with various concentrations of peptides for 1 hr and treated with 0.1nM TNF for 30 min. Nuclear extracts were prepared and NF-kB activation was analyzed by EMSA (Please refer to Takada et al, 2004 for further details). |
