CYP2B6 Induction Reporter Assay Kit
货号：
600680-1ea 基本售价：
5600.0 元 规格：
100 tests
产品信息
概述| 货号 | 600680-1ea |
| 描述 | Early screening of a drug candidate’s potential to induce or inhibit CYP2B6 is a critical step in the drug development process. Caymans CYP2B6 Induction Reporter Assay Kit is a novel method to evaluate human Constitutive Androstane Receptor 3 (hCAR3)-mediated CYP2B6 induction for the discovery of hepatic CYP inducers. Our Reverse Transfection Reporter Assays have overcome many of the disadvantages of other transfection approaches. Caymans CYP2B6 Induction Reporter Assay Kit consists of a 96-well plate coated with a transfection complex containing a Secreted Alkaline Phosphatase (SEAP) reporter regulated by the human CYP2B6 gene promoter. The complex also contains two nuclear receptor expression constructs, human Constitutive Androstane Receptor 3 (hCAR3) and human Hepatocyte Nuclear Factor 4α (HNF4α). Cells grown on the CYP2B6 Reverse Transfection Strip Plate will introduce the reporter gene and express CAR3 and HNF4α. Binding of inducer-activated receptor and transcription factors to the CYP2B6 promoter initiates a signal resulting in expression of SEAP, which is secreted into the cell culture medium. Aliquots of medium are removed at time intervals beginning at about 24 hours and SEAP activity is measured simply by adding a luminescence-based alkaline phosphatase substrate provided in the kit. The kit is simple to use and can be easily adapted to high-throughput screening for potential CAR-mediated CYP2B6 inducers. A known CYP2B6 inducer, CITCO, is included in the kit for use as a positive control. |
性能| 供应商 | Cayman |
| 运输条件 | Wet Ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 6 months |
声明| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |
