| 货号 | 10011263-100ug |
| 描述 | Two types of 15-lipoxygenase (15-LO) have been discovered and characterized, both of which metabolize arachidonic acid (AA) to produce 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE). 15-LO-1 oxygenates AA at both C-15 and C-12, whereas 15-LO-2 exclusively oxygenates C-15 of AA.1,2 Human 15-LO-2 has a molecular mass of approximately 76 kDa and exhibits approximately 40% identity to the reticulocyte 15-LO-1.2,3 Expression of 15-LO-2 appears to be restricted to prostate, lung, skin, and cornea and may play a role in the normal development of these tissues.4 The protein levels and enzymatic activity of 15-LO-2 are both down-regulated in prostate cancer compared with normal and benign prostate tissues, implicating a possible protective role for 15-LO-2 against tumor formation.4,5,6 Caymans 15-LO-2 (human recombinant) is expressed and purified from E. coli. The purity was determined using gel electrophoresis followed by coomassie staining. 15-LO-2 specific activity was established using arachidonic acid as the substrate and monitoring diene formation by measuring absorbance 236 nm. |
| 别名 | 15-LOX-2;15-LO-2; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Kuhn, H.,Barnett, J.,Grunberger, D., et al. Overexpression, purification and characterization of human recombinant 15-lipoxygenase. Biochemica et Biophysica Acta 1169(1), 80-89 (1993). 2.Brash, A.R.,Chang, M.S. and Boeglin, W.E. Discovery of a second 15S-lipoxygenase in humans. Proceedings of the National Academy of Sciences of the United States of America 94, 6148-6152 (1997). 3.Kilty, I.,Logan, A. and Vickers, P.J. Differential characteristics of human 15-lipoxygenase isozymes and novel splice variant of 15S-lipoxygenase. European Journal of Biochemistry 266, 83-93 (1999). 4.Tang, S.,Bhatia, B.,Maldonado, C.J., et al. Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells. The Journal of Biological Chemisty 277(18), 16189-16201 (2002). 5.Shappell, S.B.,Boeglin, W.E.,Olson, S.J., et al. 15-lipoxygenase-2 (15-LOX-2) is expressed in benign prostatic epithelium and reduced in prostate adenocarcinoma. American Journal of Pathology 155, 235-245 (1999). 6.Jack, G.S.,Brash, A.R.,Olson, S.J., et al. Reduced 15-lipoxygenase-2 immunostaining in prostate adenocarcinoma: Correlation with grade and expression in high-grade prostatic intraepithelial neoplasia. Human Pathology 31(9), 1146-1154 (2000). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 纯度 | ≥85% estimated by SDS-PAGE |
| 稳定性 | ≥ 6 months |
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