MitoCheck® Complex II/III Activity Assay Kit
货号:
700950-96Well 基本售价:
5390.0 元 规格:
96 wells
产品信息
概述货号 | 700950-96Well |
描述 | Complex III (CoQ cytochrome c oxidoreductase) is an essential protein for mitochondrial oxidative phosphorylation. Complex III functions as both a gatekeeper for mitochondrial respiration, and as a major source of reactive oxygen species III. It accepts electrons from complexes I and II in the form of QH2, the reduced form of the electron carrier ubiquinone. Once bound to complex III, QH2 undergoes a series of redox reactions, known as the Q-cycle. During the Q-cycle, electrons are passed from QH2 onto cytochrome cvia the Reiske iron-sulfur protein and cytochrome c1 resulting in the translocation of 4H+ and the generation of 02•-. Caymans MitoCheck® Complex II/III Activity Assay measures the reduction of excess cytochrome c (550 nm absorbance) as catalyzed by complex III. This assay is coupled to succinate co-enzyme Q oxidoreductase (complex II) for the generation of QH2. Due to the dependence on complex II activity, a counterscreen for complex II activity (Item No. 700940) should be performed in order to truly measure complex III activity. For the use of this kit with other types of tissue mitochondria, please see Caymans MitoCheck® Mitochondrial (Tissue) Isolation Kit (Item No. 701010). |
性能供应商 | Cayman |
运输条件 | Dry ice in continental US; may vary elsewhere |
存放说明 | -80 |
稳定性 | ≥ 1 year |
声明本官网所有报价均为常温或者蓝冰运输价格,如有产品需要干冰运输,需另外加收干冰运输费。 |