| 货号 | 16381-25mg |
| 描述 | 7-methyl-6-Thioguanosine is a chromophoric substrate that is used to quantify inorganic phosphate (Pi). In the presence of purine nucleoside phosphorylase, it reacts with Pi to produce 7-methyl-6-thioguanine and ribose 1-phosphate, with the increase in 7-methyl-6-thioguanine followed by continuous spectrophotometry at an absorbance of 355-360 nm.1 7-methyl-6-Thioguanosine is also used to measure changes in Pi levels in coupled enzyme systems, including assays of the activities of protein phosphatases, phosphorylase kinases, GTPases, and ATPases.1,2,3,4 |
| 别名 | MESG; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Webb, M.R. A continuous spectrophotometric assay for inorganic phosphate and for measuring phosphate release kinetics in biological systems. Proceedings of the National Academy of Sciences of the United States of America 89(11), 4884-4887 (1992). 2.Cheng, Q.,Wang, Z.X. and Killilea, S.D. A continuous spectrophotometric assay for protein phosphatases. Analytical Biochemistry 310(2), 156-162 (2002). 3.Wang, Z.X.,Cheng, Q. and Killilea, S.D. A continuous spectrophotometric assay for phosphorylase kinase. Analytical Biochemistry 230(1), 55-61 (1995). 4.Tan, Y.C.,Wu, W.N.,Zheng, Y., et al. Characterization of the interactions between the small GTPase RhoA and its guanine nucleotide exchange factors. Analytical Biochemistry 310(2), 156-162 (2014). | |
| 运输条件 | Room temperature in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 纯度 | ≥85% |
| 计算分子量 | 313.3 |
| 分子式 | C11H15N5O4S |
| CAS号 | 55727-10-1 |
| 稳定性 | ≥ 2 years |
| 本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。 |