| 货号 | 15137-100ug |
| 描述 | Eukaryotic translation is a complex biochemical process requiring several initiation factors. The first step in the initiation of mRNA translation involves binding of the small ribosomal subunit to mRNA.1 The central component in the recruitment of the pre-initiation complex during translation is the eukaryotic initiation factor 4F (eIF4F) complex, composed of eIF4A, eIF4E, and eIF4G1/eIF4G3.2 The 5’-terminal end of eukaryotic mRNA has a common cap structure that is important for stabilizing mRNA and ribosome binding.3 The eIF4E component binds specifically to the mRNA cap structure to initiate protein synthesis by recognizing methyl-7-guanosine.2,4 eIFA is a DEAD-box helicase, responsible for unwinding the mRNA, while eIF4G acts as a scaffold protein with binding sites for the poly(A)-binding protein and eIF3. eIF4G also possesses central and C-terminal eIF4A binding sites and a C-terminal binding site for the protein kinase Mnk1 (mitogen-activated protein kinase-interacting kinase).5 Mnk1 phosphorylates eIF4E at Ser209, which is believed to be essential for eIF4E’s oncogenic potential.6,7,8 Further, overexpression of eIF4e is seen in many human malignancies, including breast, colon, head and neck carcinomas, and others.9,10 |
| 别名 | eIF-4F 25 kDa subunit;Eukaryotic Translation Initiation Factor 4E;mRNA Cap-binding Protein; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Tomoo, K.,Shen, X.,Okabe, K., et al. Crystal structures of 7-methylguanosine 5-triphosphate (m7GTP)- and P1-7-methylguanosine-P3-adenosine-5,5-triphosphate (m7GpppA)-bound human full-length eukaryotic initiation factor 4E: Biological importance of the C-terminal flexible region. Biochemistry Journal 362(pt 3), 539-544 (2002). 2.Marcotrigiano, J.,Gingras, A.C.,Sonenberg, N., et al. Cocrystal structure of the messenger RNA 5 cap-binding protein (eIF4E) bound to 7-methyl-GDP. Cell 89(6), 951-961 (1997). 3.Morino, S.,Hazama, H.,Ozaki, M., et al. Analysis of the mRNA cap-binding ability of human eukaryotic initiation factor-4E by use of recombinant wild-type and mutant forms. European Journal of Biochemistry 239(3), 597-601 (1996). 4.Haghighat, A. and Sonenberg, N. eIF4G dramatically enhances the binding of eIF4E to the mRNA 5-cap structure. The Journal of Biological Chemisty 272(35), 21677-21680 (1997). 5.Schütz, P.,Bumann, M.,Oberholzer, A.E., et al. Crystal structure of the yeast eIF4A-eIF4G complex: An RNA-helicase controlled by protein-protein interactions. Proceedings of the National Academy of Sciences of the United States of America 105(28), 9564-9569 (2008). 6.Shveygert, M.,Kaiser, C.,Bradrick, S.S., et al. Regulation of eukaryotic initiation factor 4E (eIF4E) phosphorylation by mitogen-activated protein kinase occurs through modulation of Mnk1-eIF4G interaction. Mol.Cell Biol. 30(21), 5160-5167 (2010). 7.Silva, R.L. and Wendel, H.G. MNK, EIF4E and targeting translation for therapy. Cell Cycle 7(5), 553-555 (2008). 8.Furic, L.,Rong, L.,Larsson, O., et al. eIF4E phosphorylation promotes tumorigenesis and is associated with prostate cancer progression. Proceedings of the National Academy of Sciences of the United States of America 107(32), 14134-14139 (2010). 9.Carroll, M. and Borden, K.L. The oncogene eIF4E: Using biochemical insights to target cancer. J.Interferon Cytokine Res. 33(5), 227-238 (2013). 10.Yin, X.,Kim, R.H.,Sun, G., et al. Overexpression of eukaryotic initiation factor 4E is correlated with increased risk for systemic dissemination in node-positive breast cancer patients. J.Am.Coll.Surg. (2014). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 纯度 | ≥90% estimated by SDS-PAGE |
| 稳定性 | ≥ 1 year |
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