| 货号 | 10782-100ug |
| 描述 | Gcn5 and PCAF are highly homologous members of the Gcn5-related N-acetyltransferase (GNAT) superfamily of N-acetyltransferases involved in histone acetylation.1 Gcn5 and PCAF contain a highly conserved central core with divergent N- and C-terminal ends.2 Gcn5/PCAF are enzymatic subunits that exist in a mutually exclusive manner as part of the mammalian SAGA and ATAC complexes.3,4 Recombinant Gcn5 preferentially acetylates lysine 14 on histone H3 in vitro. Recombinant Gcn5 alone is unable to acetylate nucleosomal core histone substrates. Acetylation of the nucleosomal histones requires that Gcn5 be a part of either the multisubunit SAGA or ATAC protein complexes.5 The multisubunit Gcn5/PCAF-containing complexes have a broad substrate specificity, including H3K9, H3K18, H4K8, and H4K16, as well as additional sites on histone H2B.3 |
| 别名 | General control of amino acid synthesis protein 5-like 2;KAT2A;Lysine acetyltransferase 2A;STAF97; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Vetting, M.W.,S.de Carvalho, L.P.,Yu, M., et al. Structure and functions of the GNAT superfamily of acetyltransferases. Archives of Biochemistry and Biophysics 433(1), 212-226 (2005). 2.Trievel, R.C.,Rojas, J.R.,Sterner, D.E., et al. Crystal structure and mechanism of histone acetylation of the yeast GCN5 transcriptional coactivator. Proceedings of the National Academy of Sciences of the United States of America 96(16), 8931-8936 (1999). 3.Roth, S.Y.,Denu, J.M. and Allis, C.D. Histone acetyltransferases. Annual Reviews of Biochemistry 70, 81-120 (2001). 4.Wang, Y.L.,Faiola, F.,Xu, M., et al. Human ATAC is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein. The Journal of Biological Chemisty 283(49), 33808-33815 (2008). 5.Grant, P.A.,Sterner, D.E.,Duggan, L.J., et al. The SAGA unfolds: Convergence of transcription regulators in chromatin-modifying complexes. Trends in Cell Biology 8(5), 193-197 (1998). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 纯度 | Purified by affinity chromatography |
| 稳定性 | ≥ 6 months |
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