| 货号 | 11237-100ug |
| 描述 | Methylation of lysine residues in core histones plays a critical role in regulating gene expression.1 Jumonji domain containing 2E (JMJD2E) catalyzes the demethylation of histone H3 at lysince residue 9.2 Like other JmjC protein hydroxylase family members, JMJD2E is an α-ketoglutarate-dependent Fe (II) oxygenase.3 Purification of Fe-dependent JmjC family members by IMAC can result in displacement of the catalytic iron and decreased activity, therefore this Strep-tagged protein is purified by affinity chromatography using Strep-Tactin coated resin.4 Because of their implication in cancer cell growth, jumonji C domain-containing histone demethylase inhibitors may have the capacity to be anticancer agents.1 |
| 别名 | Jumonji Domain Containing 2E;KDM4D-Like; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Hamada, S.,Kim, T.D.,Suzuki, T., et al. Synthesis and activity of N-oxalylglycine and its derivatives as Jumonji C-domain-containing histone lysine demethylase inhibitors. Bioorganic & Medicinal Chemistry Letters 19, 2852-2855 (2009). 2.Kouzarides, T. Chromatin modifications and their function. Cell 128, 693-705 (2007). 3.Couture, J.F.,Collazo, E.,Ortiz-Tello, P.A., et al. Specificity and mechanism of JMJD2A, a trimethyllysine-specific histone demethylase. Nature Structural & Molecular Biology 14(8), 689-695 (2007). 4.Krishnan, S.,Collazo, E.,Ortiz-Tello, P.A., et al. Purification and assay protocols for obtaining highly active Jumonji C demethylases. Analytical Biochemistry 420, 48-53 (2012). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 纯度 | ≥75% estimated by SDS-PAGE |
| 稳定性 | ≥ 6 months |
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