| 货号 | 15563-100ug |
| 描述 | Catechol-O-methyltransferase (COMT) plays an important role in normal brain function and has been implicated in human disorders, such as Parkinsons disease. Using S-adenosyl-L-methionine (SAM) as a methyl donor, COMT methylates catechol substrates, which include neurotransmitters, such as dopamine, norepinephrine, epinephrine, and catecholestrogens; ascorbic acid; and carcinogenic flavonoids.1 There are two classes of COMT, the soluble cytosolic form (S-COMT), and the rough endoplasmic reticulum membrane bound form (MB-COMT).2 Both forms are coded by a single gene using two separate promoters.2 Cayman’s COMT is the soluble S-COMT isoform and is composed of 221 amino acids, whereas MB-COMT has an additional 50 amino acid residues, including 21 hydrophobic amino acid residues that compose the membrane anchor region.3 While S-COMT is predominate in most tissues, MB-COMT is found in higher concentrations in the brain.4 A common variant in exon 4 of the COMT gene leads to a substitution of valine by methionine, Val158Met, which affects the stability of COMT and may diminish activity by 50% in the human brain. Since COMT is one of the primary regulators of prefrontal cortex dopamine levels, it plays an important role in cognition.5 |
| 别名 | COMT;Pyrocatechol-O-methyltransferase;S-Adenosyl-L-methionine:Catechol O-Methyltransferase; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Lundström, K.,Tenhunen, J.,Tilgmann, C., et al. Cloning, expression and structure of catechol-O-methyltransferase. Biochimica et Biophysica Acta 1251(1), 1-10 (1995). 2.Tilgmann, C. and Ulmanen, I. Purification methods of mammalian catechol-O-methyltransferases. J.Chromatogr.B.Biomed.Appl. 684(1-2), 147-161 (1996). 3.Pedro, A.Q.,Bonifácio, M.J.,Queiroz, J.A., et al. A novel prokaryotic expression system for biosynthesis of recombinant human membrane-bound catechol-O-methyltransferase. Journal of Biotechnology 156(2), 141-146 (2014). 4.Ma, Z.,Liu, H. and Wu, B. Structure-based drug design of catechol-O-methyltransferase inhibitors for CNS disorders. British Journal of Clinical Pharmacology 77(3), 410-420 (2014). 5.Dickinson, D. and Elvevåg, B. Genes, cognition and brain through a COMT lens. Neuroscience 164(1), 72-87 (2009). | |
| 运输条件 | Dry ice in continental US; may vary elsewhere |
| 存放说明 | -80 |
| 纯度 | ≥70% estimated by SDS-PAGE |
| 稳定性 | ≥ 6 months |
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