| 货号 | 765001-96Well |
| 描述 | Phospholipase A2(PLA2) catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid.1 The release of arachidonic acid from membrane phospholipids by PLA2 is believed to be a key step in the control of eicosanoid production within the cell.2 The Cayman Chemical secretory PLA2(sPLA2) Assay kit provides an accurate and convenient method for measurement of sPLA2 activity. This assay uses the 1,2-dithio analog of diheptanoyl phosphatidylcholine which serves as a substrate for most PLA2s (for example, bee and cobra venoms, pancreatic, etc.) with the exception of cytosolic PLA2.3,4 Upon hydrolysis of the thio ester bond at the sn-2 position by PLA2, free thiols are detected using DTNB (5,5-dithio-bis-(2-nitrobenzoic acid)). |
| 别名 | Secretory Phospholipase A2 (Type IIA) Assay Kit; |
| 供应商 | Cayman |
| 应用文献 | |
| 1.Dennis, E.A. Phospholipases. Enzymes XVI, 307-353 (1983). 2.Dennis, E.A. Diversity of group types, regulation, and function of phospholipase A2. The Journal of Biological Chemisty 269, 13057-13060 (1994). 3.Hendrickson, H.S.,Hendrickson, E.K., and Dybvig, R.H. Chiral synthesis of a dithiolester analog of phosphatidylcholine as a substrate for the assay of phospholipase A2. Journal of Lipid Research 24, 1532-1537 (1983). 4.Reynolds, L.J.,Hughes, L.L., and Dennis, E.A. Analysis of human synovial fluid phospholipase A2 on short chain phosphatidylcholine-mixed micelles: Development of a spectrophotometric assay suitable for a microtiterplate reader. Analytical Biochemistry 204, 190-197 (1992). | |
| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 1 year |
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