Hydrogen Peroxide Cell-Based Assay Kit
货号：
600050-480Well 基本售价：
4256.0 元 规格：
480 wells
产品信息
概述| 货号 | 600050-480Well |
| 描述 | Reactive oxygen species, such as hydrogen peroxide (H2O2) and superoxide are generated by phagocytes and participate in damaging invading miccroorganisms or other biologic targets.1 Caymans H2O2 Cell-Based Assay Kit provides a simple fluorometric method for the sensitive quantitation of extracellular H2O2 produced by cultured cells. H2O2 is detected using ADHP (10-acetyl-3,7-dihydroxyphenoxazine), a highly sensitive and stable probe for H2O2.2 In the presence of horseradish peroxidase, ADHP reacts with H2O2 with a 1:1 stoichiometry to produce highly fluorescent resorufin.3 Resorufin fluorescence can be read using an excitation wavelength between 530-560 nm and an emission wavelength of 590 nm. Catalase, an H2O2 scavenger, is included in the kit for checking specificity of the assay. |
| 别名 | H2O2 Fluorometric Assay Kit; |
性能| 供应商 | Cayman |
| 应用文献 |
1.Rosen, G.M.,Pou, S.,Ramos, C.L., et al. Free radicals and phagocytic cells. The FASEB Journal 9, 200-209 (1995).
2.Zhou, M.,Diwu, Z.,Panchuk-Voloshina, N., et al. A stable nonfluorescent derivative of resorufin for the fluorometric determination of trace hydrogen peroxide: Applications in detecting the activity of phagocyte NADPH oxidase and other oxidases. Analytical Biochemistry 253, 162-168 (1997).
3.Amundson, D.M. and Zhou, M. Fluorometric method for the enzymatic determination of cholesterol. Journal of Biochemical and Biophysical Methods 38, 43-52 (1999).
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| 运输条件 | Wet ice in continental US; may vary elsewhere |
| 存放说明 | -20 |
| 稳定性 | ≥ 6 months |
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